Mulder H, Uddman R, Moller K, Zhang Y Z, Ekblad E, Alumets J, Sundler F
Department of Medical Cell Research, Malmö General Hospital, Lund, Sweden.
Neuroscience. 1994 Nov;63(1):307-12. doi: 10.1016/0306-4522(94)90025-6.
Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel vasoactive intestinal peptide (VIP)-like peptide isolated from ovine hypothalami. The presence of PACAP-like immunoreactivity was recently demonstrated in nerve cell bodies of sensory ganglia in the rat. Since PACAP belongs to a large family of chemically related neuropeptides, we have, in the present study, tried to establish the synthesis of PACAP in neurons of sensory ganglia, using in situ hybridization with a 35S-labelled oligonucleotide probe complementary to PACAP mRNA. The expression of PACAP was compared to that of calcitonin gene-related peptide (CGRP) using a radiolabelled CGRP oligonucleotide probe. The PACAP probe labelled small to medium-sized neurons in the trigeminal ganglion and dorsal root ganglia at different levels, indicating the presence of PACAP mRNA. The CGRP probe labelled nerve cell bodies of varying size, outnumbering those labelled by the PACAP probe. In dorsal root ganglia, cells expressing PACAP constituted c. 10% and those expressing CGRP 46% of the total number of nerve cell bodies. Expression of PACAP was seen in a small subpopulation of cells expressing CGRP. We conclude that PACAP is synthesized in a subpopulation of neurons of sensory ganglia in the rat. Therefore, the recently described effects of PACAP--cutaneous vasodilation, potentiation of oedema formation and depression of nociceptive spinal reflexes--may be physiological and related to neurogenic inflammation and modulation of pain transmission.
垂体腺苷酸环化酶激活多肽（PACAP）是一种从绵羊下丘脑分离出的新型血管活性肠肽（VIP）样肽。最近在大鼠感觉神经节的神经细胞体中证实了PACAP样免疫反应性的存在。由于PACAP属于一大类化学相关的神经肽家族，在本研究中，我们试图通过与与PACAP mRNA互补的35S标记寡核苷酸探针进行原位杂交，来确定感觉神经节神经元中PACAP的合成情况。使用放射性标记的降钙素基因相关肽（CGRP）寡核苷酸探针，将PACAP的表达与CGRP的表达进行比较。PACAP探针标记了不同水平的三叉神经节和背根神经节中小到中等大小的神经元，表明存在PACAP mRNA。CGRP探针标记了大小各异的神经细胞体，其数量超过了PACAP探针标记的细胞。在背根神经节中，表达PACAP的细胞约占神经细胞体总数的10%，而表达CGRP的细胞占46%。在表达CGRP的一小部分细胞亚群中可见PACAP的表达。我们得出结论，PACAP是在大鼠感觉神经节的一部分神经元亚群中合成的。因此，最近描述的PACAP的作用——皮肤血管舒张、水肿形成增强和伤害性脊髓反射抑制——可能是生理性的，与神经源性炎症和疼痛传递的调节有关。
