Herbers J, Schullerus D, Müller H, Kenck C, Chudek J, Weimer J, Bugert P, Kovacs G
Department of Urology, Ruprecht-Karls-University, Heidelberg, Germany.
Genes Chromosomes Cancer. 1997 May;19(1):29-35.
We examined 88 nonpapillary renal cell carcinomas for allelic loss at chromosome arm 14q and correlated the results to size, grade, and stage of these tumors. Fourteen highly polymorphic microsatellite markers on the long arm of chromosome 14 were used for deletion mapping. Loss of heterozygosity (LOH) at the smallest overlapping segment of 14q24.2-qter was seen in 42 of 88 tumors. There was no significant correlation between frequency of 14q LOH and size of tumors (P = 0.11). LOH was frequently seen in grade 2 and 3 tumors (55% and 73%, respectively) and in stage III and IV tumors (53% and 80%, respectively). We found a significant correlation between chromosome arm 14q LOH and nuclear grade (P < 0.001) and stage (P < 0.001) of tumors. These observations indicate the presence of a tumor-suppressor gene at chromosome segment 14q24.2-qter and demonstrate the usefulness of microsatellite analysis for assessing the possible clinical outcome of nonpapillary renal cell carcinomas.
我们检测了88例非乳头状肾细胞癌14号染色体臂上等位基因缺失情况，并将结果与这些肿瘤的大小、分级和分期进行关联分析。利用位于14号染色体长臂上的14个高度多态性微卫星标记进行缺失定位。在88例肿瘤中，有42例在14q24.2-qter最小重叠片段出现杂合性缺失（LOH）。14q LOH频率与肿瘤大小之间无显著相关性（P = 0.11）。LOH在2级和3级肿瘤中常见（分别为55%和73%），在III期和IV期肿瘤中也常见（分别为53%和80%）。我们发现14号染色体臂LOH与肿瘤的核分级（P < 0.001）和分期（P < 0.001）之间存在显著相关性。这些观察结果表明在染色体片段14q24.2-qter存在一个肿瘤抑制基因，并证明了微卫星分析在评估非乳头状肾细胞癌可能的临床结局中的有用性。
