Liu L, Dai Y, Moor R M
College of Animal Science and Technology, Beijing Agricultural University, People's Republic of China.
Mol Reprod Dev. 1997 Jun;47(2):191-9. doi: 10.1002/(SICI)1098-2795(199706)47:2<191::AID-MRD10>3.0.CO;2-3.
Experiments were designed to identify the extent to which follicle cells and hormones contribute to the developmental competence of porcine oocytes matured in vitro. Oocyte-cumulus complexes were collected from ovaries by dissection and cultured in 2 ml of TCM199-based medium in 5% CO2 in humidified air at 38.5 degrees C. This basic maturation system was supplemented, for either the first 24 hr only or for the 48-hr culture period, with 1) everted follicle shell alone, 2) gonadotrophic hormones alone, or 3) both follicle shells and hormones. The effect of these treatments was evaluated on 1) meiotic maturation rates, 2) the capacity of matured eggs to undergo activation and early cleavage, and 3) changes to the profile of proteins secreted into the culture medium. The results showed that 1) supplementation with either follicle shell or hormones alone increased the rates of meiotic maturation over the nonsupplemented control group, and 2) combined follicle shell and hormonal supplementation yielded the highest rates for maturation, activation, and cleavage but only when hormonal supplementation was removed after the first 24 hr of culture. Proteins of 30, 37, 45, and 46 kD, but of unknown function, were secreted during the first 24 hr into the culture medium in groups supplemented with follicle shells. The addition of hormones did not affect this pattern of secreted proteins. It is possible that some secreted proteins may act to facilitate full maturation of pig oocytes.
设计实验以确定卵泡细胞和激素对体外成熟的猪卵母细胞发育能力的贡献程度。通过解剖从卵巢收集卵母细胞-卵丘复合体，并在38.5摄氏度、含5%二氧化碳的湿润空气中，于2毫升基于TCM199的培养基中培养。这个基本的成熟系统在仅前24小时或48小时培养期内进行补充，补充物为：1）单独的翻转卵泡壳；2）单独的促性腺激素；3）卵泡壳和激素两者。评估这些处理对以下方面的影响：1）减数分裂成熟率；2）成熟卵激活和早期卵裂的能力；3）分泌到培养基中的蛋白质谱的变化。结果表明：1）单独补充卵泡壳或激素均使减数分裂成熟率高于未补充的对照组；2）卵泡壳和激素联合补充产生最高的成熟、激活和卵裂率，但前提是在培养的前24小时后去除激素补充。在补充卵泡壳的组中，30、37、45和46千道尔顿的蛋白质（功能未知）在最初24小时分泌到培养基中。激素的添加不影响这种分泌蛋白质的模式。一些分泌的蛋白质可能有助于猪卵母细胞的完全成熟。
