University of Monastir, Faculty of Dental Medecine, Laboratory for Research on Biologically Compatible Compounds, LR01SE17, 5019, Monastir, Tunisia.
Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue TaherHadded, 5000, Monastir, Tunisia.
Environ Sci Pollut Res Int. 2020 Mar;27(8):7957-7966. doi: 10.1007/s11356-019-07395-x. Epub 2019 Dec 31.
The increased use of pesticides is the origin of multiple damages to the environment and to humans; thus, the search for new strategies to reduce or even protect the toxic effects caused by these synthetic products became a necessity. In this context, our study attempted to evaluate the protective effects of fennel essential oil (FEO), the main essential oil extracted from Faeniculum vulgare Mill., a plant with aromatic, flavorful, and medicinal uses, against toxicity induced by an insecticide-triflumuron (TFM)-in human carcinoma cells (HCT116). Our methodological approach consists of the cytotoxicity assay starting with the cell viability test, the ROS generation, the malondialdehyde (MDA) production, the DNA fragmentation, and the measurement of some antioxidant enzymes activities such as catalase (CAT) and superoxide dismutase (SOD). Also, we measured the mitochondrial transmembrane potential. The outcome of the current study showed clearly that after 2 h of HCT 116 cell pretreatment with FEO, there were increase in cell viability, reduction in ROS generation, and modulation in CAT and SOD activities induced by TFM. In the same manner, significant decreases in MDA levels were found. Mainly, the results indicated a perceptible decrease in DNA damages and a significant reduction in the mitochondrial membrane potential loss. Our work demonstrates that FEO can be an important protector against toxic effects induced by TFM in HCT 116 cells.
杀虫剂的大量使用是对环境和人类造成多种损害的根源；因此，寻找新的策略来减少甚至保护这些合成产品造成的毒性影响成为当务之急。在这种情况下，我们的研究试图评估茴香精油（FEO）的保护作用，FEO 是从植物茴香（Faeniculum vulgare Mill.）中提取的主要精油，该植物具有芳香、美味和药用用途，以对抗杀虫剂噻虫隆（TFM）在人结肠癌细胞（HCT116）中引起的毒性。我们的方法学方法包括从细胞活力测试开始的细胞毒性测定、ROS 生成、丙二醛（MDA）产生、DNA 片段化以及一些抗氧化酶活性（如过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的测量。此外，我们还测量了线粒体跨膜电位。目前研究的结果清楚地表明，在用 FEO 预处理 HCT 116 细胞 2 小时后，细胞活力增加，TFM 诱导的 ROS 生成减少，CAT 和 SOD 活性发生调节。同样，MDA 水平也显著降低。主要是，结果表明 DNA 损伤明显减少，线粒体膜电位损失显著降低。我们的工作表明，FEO 可以作为一种重要的保护剂，防止 TFM 对 HCT 116 细胞产生的毒性影响。
