Mori Y, Hiraki Y, Shukunami C, Kakudo S, Shiokawa M, Kagoshima M, Mano H, Hakeda Y, Kurokawa T, Suzuki F, Kumegawa M
Department of Oral Anatomy, Meikai University School of Dentistry, Sakado, Saitama, Japan.
FEBS Lett. 1997 Apr 14;406(3):310-4. doi: 10.1016/s0014-5793(97)00291-3.
We previously reported the isolation of the cartilage-derived growth promoting factors chondromodulin-I (ChM-I) and chondromodulin-II (ChM-II) from fetal bovine epiphyseal cartilage. Both of these factors stimulate the growth and matrix formation of chondrocytes in vitro. In the present study, we found that ChM-I and ChM-II stimulated the proliferation of clonal mouse osteoblastic MC3T3-E1 cells as well as primary mouse osteoblasts in culture. Unlike other known growth factors, these factors did not support the proliferation of fibroblasts. Concomitantly with growth stimulation of osteoblasts, there was a reduction of alkaline phosphatase (ALP) activity in the cells, the expression of the differentiated phenotype. These results suggest that epiphyseal cartilage may play a functional role in longitudinal bone growth by production of these unique growth-promoting factors.
我们之前报道过从胎牛骺软骨中分离出软骨衍生生长促进因子软骨调节素-I（ChM-I）和软骨调节素-II（ChM-II）。这两种因子在体外均能刺激软骨细胞的生长和基质形成。在本研究中，我们发现ChM-I和ChM-II能刺激克隆化小鼠成骨细胞MC3T3-E1细胞以及培养中的原代小鼠成骨细胞的增殖。与其他已知生长因子不同，这些因子不支持成纤维细胞的增殖。在刺激成骨细胞生长的同时，细胞中碱性磷酸酶（ALP）活性降低，即分化表型的表达降低。这些结果表明，骺软骨可能通过产生这些独特的生长促进因子在长骨生长中发挥功能性作用。
