Mato T, Masuko K, Misaki Y, Hirose N, Ito K, Takemoto Y, Izawa K, Yamamori S, Kato T, Nishioka K, Yamamoto K
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Int Immunol. 1997 Apr;9(4):547-54. doi: 10.1093/intimm/9.4.547.
It has been proposed that T cell activation may play an important role in the pathogenesis of systemic lupus erythematosus (SLE). In order to examine this hypothesis, we assessed the whole degree of clonal accumulation of T cells using RT-PCR and subsequent single-strand conformation polymorphism (SSCP) analysis. The analysis of the beta chain of the TCR revealed little clonotypic T cell expansion in the peripheral blood of lupus patients in remission, whereas in patients with active disease many dominant T cell clonal expansions without any distinct V beta bias were observed. The alteration in the number of T cell clones correlated well with disease activities, since these T cell expansions decreased as patients had improved. Furthermore, similar but more intense accumulations of T cell clones were found in pleural and pericardial effusions of patients with lupus serositis. Some of these identical expanded clonotypes were observed irrespective of the lesions and the times of sampling, and some of them were identical to those observed in the peripheral blood. These results suggest that the T cell clonal expansions correlate with the disease activities and that the expansion might contribute to the pathogenic lesion formation in SLE.
有人提出，T细胞活化可能在系统性红斑狼疮（SLE）的发病机制中起重要作用。为了验证这一假设，我们使用逆转录聚合酶链反应（RT-PCR）和随后的单链构象多态性（SSCP）分析评估了T细胞克隆积累的整体程度。对T细胞受体β链的分析显示，缓解期狼疮患者外周血中几乎没有克隆型T细胞扩增，而在疾病活动期的患者中，观察到许多显性T细胞克隆扩增，且没有任何明显的Vβ偏向。T细胞克隆数量的变化与疾病活动密切相关，因为随着患者病情好转，这些T细胞扩增减少。此外，在狼疮性浆膜炎患者的胸腔和心包积液中发现了类似但更强烈的T细胞克隆积累。无论病变和取样时间如何，都观察到一些相同的扩增克隆型，其中一些与外周血中观察到的相同。这些结果表明，T细胞克隆扩增与疾病活动相关，并且这种扩增可能有助于SLE中致病病变的形成。
