Department of Chemistry Jamia Millia Islamia (Central University), New Delhi 110025, India.
Chirality. 2010 Jan;22(1):24-8. doi: 10.1002/chir.20697.
Sixteen beta-adrenergic antagonists namely acebutalol, alprenolol, atenolol, bisoprolol, bopindolol, bufurolol, carazolol, celiprolol, indenolol, metaprolol, nebivolol, oxprenolol, practolol, propranolol, tertalol, and timolol, and two beta-adrenergic agonists namely cimeterol and clenbuterol were resolved on AmyCoat (150 x 46 mm, 3 microm size of silica particle) by using (85:15:0.1, v/v/v), (90:10:0.1, v/v/v), and (95:05:0.1, v/v/v) combinations of n-heptane, ethanol, and diethylamine solvents, respectively. The flow rates used were 0.5, 1.0, 2.0, and 3.0 ml/min with detection at 225 nm. The values of capacity, separation, and resolution factors ranged from 0.38 to 19.70, 1.08-2.33, and 1.0 and 4.50, respectively. The maximum and minimum resolutions were achieved for celiprolol and bufurolol, respectively. The chiral recognition mechanisms were also discussed. The values of validation parameters were calculated.
16 种β-肾上腺素拮抗剂，即乙酰丁醇、阿普洛尔、阿替洛尔、比索洛尔、布福洛尔、卡唑洛尔、塞利洛尔、美托洛尔、奈比洛尔、纳多洛尔、氧烯洛尔、普萘洛尔、丙萘洛尔、特他林和噻吗洛尔，以及两种β-肾上腺素激动剂，即西美特洛尔和克仑特罗，在 AmyCoat（150x46mm，3μm 大小的硅胶颗粒）上通过使用 n-庚烷、乙醇和二乙胺溶剂的（85:15:0.1，v/v/v）、（90:10:0.1，v/v/v）和（95:05:0.1，v/v/v）组合进行了分离。流速分别为 0.5、1.0、2.0 和 3.0ml/min，检测波长为 225nm。容量、分离和分辨率因子的值范围分别为 0.38-19.70、1.08-2.33 和 1.0-4.50。最大和最小分辨率分别为塞利洛尔和布福洛尔。还讨论了手性识别机制。验证参数的值也进行了计算。
