Wagenknecht D R, Green K M, McIntyre J A
Center for Reproduction and Transplantation Immunology, Methodist Hospital of Indiana, Indianapolis, USA.
Am J Reprod Immunol. 1997 Jan;37(1):1-6. doi: 10.1111/j.1600-0897.1997.tb00186.x.
The influence of HLA sharing on pregnancy outcome is controversial. In renal transplantation, HLA-DQB1 donor-recipient mismatches have been shown beneficial for long-term transplant success. Since pregnancy is defined as Nature's allograft, we investigated the relevance of HLA-DQ mismatching in normal reproducing couples compared to couples experiencing RSA.
Unexplained RSA couples referred to our laboratory for immunological testing were classified by immunological findings and obstetrical history. Primary RSA couples shared > or = 2 HLA-A, B, or DR antigens, had no cytotoxic anti-paternal antibodies, and no gestation beyond 20 weeks. Secondary RSA couples had cytotoxic anti-paternal antibodies and RSA after a live birth. HLA-DQA1 and DQB1 alleles were identified by PCR-SSP.
No differences in DQA1 and DQB1 mismatch were observed among RSA patients and controls. DQA1-DQB1 haplotype mismatches were not different among the three groups of couples.
In contrast to renal transplant, HLA-DQ incompatibility did not differ among RSA couples compared with successful reproducing couples.
人类白细胞抗原（HLA）匹配对妊娠结局的影响存在争议。在肾移植中，HLA - DQB1供体 - 受体错配已被证明对长期移植成功有益。由于妊娠被定义为自然的同种异体移植，我们研究了与反复自然流产（RSA）夫妇相比，正常生育夫妇中HLA - DQ错配的相关性。
因免疫检测转诊至我们实验室的不明原因RSA夫妇，根据免疫检查结果和产科病史进行分类。原发性RSA夫妇共享≥2个HLA - A、B或DR抗原，无细胞毒性抗父抗体，且妊娠未超过20周。继发性RSA夫妇有细胞毒性抗父抗体且在活产后发生RSA。通过聚合酶链反应 - 序列特异性引物（PCR - SSP）鉴定HLA - DQA1和DQB1等位基因。
RSA患者与对照组之间在DQA1和DQB1错配方面未观察到差异。三组夫妇之间DQA1 - DQB1单倍型错配无差异。
与肾移植不同，与成功生育夫妇相比，RSA夫妇之间的HLA - DQ不相容性并无差异。
