Check J H, Arwitz M, Gross J, Peymer M, Szekeres-Bartho J
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, USA.
Am J Reprod Immunol. 1997 Jan;37(1):17-20. doi: 10.1111/j.1600-0897.1997.tb00188.x.
To determine if allogenic stimulation from leukocyte immunization (LI) can increase the production of an immunomodulatory protein called progesterone induced blocking factor (PIBF) by CD8+ T-lymphocytes.
The study group consisted of 35 women, 29 who failed to conceive after repeated embryo transfers (ETs) and six with recurrent spontaneous abortion (RSA). The women underwent LI using the the male partner's blood as the source of leukocytes. Progesterone induced blocking factor was measured pre- and post-LI with an immunocytochemistry method using a PIBF-specific polyclonal antibody.
The mean percentage of lymphocytes expressing PIBF, as well as the percentage of cases of whose PIBF level increased to 1% or more, was significantly higher post-LI, Similarly post-LI, there was a significantly lower percentage of zero PIBF levels.
Leukocyte immunization causes an increase in PIBF in many cases. Possibly the improved pregnancy outcome in immunized patients with RSA or previous failure to conceive with in vitro fertilization may be partially or possibly completely explained by its stimulatory effect on PIBF.
确定白细胞免疫（LI）产生的同种异体刺激是否能增加CD8 + T淋巴细胞产生一种名为孕酮诱导阻断因子（PIBF）的免疫调节蛋白。
研究组由35名女性组成，其中29名在反复胚胎移植（ET）后未能受孕，6名有复发性自然流产（RSA）。这些女性使用男性伴侣的血液作为白细胞来源进行白细胞免疫。使用PIBF特异性多克隆抗体，通过免疫细胞化学方法在白细胞免疫前后测量孕酮诱导阻断因子。
白细胞免疫后，表达PIBF的淋巴细胞的平均百分比以及PIBF水平增加到1%或更高的病例百分比显著更高。同样在白细胞免疫后，PIBF水平为零的百分比显著更低。
白细胞免疫在许多情况下会导致PIBF增加。免疫治疗的复发性自然流产患者或先前体外受精未能受孕的患者妊娠结局改善，可能部分或完全是由于其对PIBF的刺激作用。
