DePalma L
Department of Pathology, George Washington University Medical Center, Washington, DC, USA.
Biotech Histochem. 1996 Mar;71(2):57-60. doi: 10.3109/10520299609117133.
IRon stains are often used for bone marrow core biopsies obtained by needle biopsy of the iliac crest. Because bone must be decalcified by brief treatment with acid, it is possible that an undetermined amount of stainable histiocytic iron may be lost. A study was carried out to determine whether decalcification results in loss of histiocytic iron and the effects of fixatives and the recovery of histiocytic iron in decalcified bone marrow tissue. Aspirates of bone marrow were stained for iron with Prussian blue. Because aspirate material does not require decalcification, it served as a control for the study. One hundred bone marrow biopsies and accompanying aspirates from 100 adult subjects were evaluated. Fifty bone marrow biopsies were fixed using a fixative containing mercuric chloride (B-5) and the remaining 50 were fixed in zinc-formalin. Histiocytic iron was graded as minimal, moderate or marked depending on whether less than 5, 6-10, or more than 10 iron positive histiocytes, respectively, were observed. When histiocytic iron was markedly present in aspirate material, at least moderate amounts of stainable iron were found in 22 of 25 B-5 fixed and 21 of 25 zinc-formalin fixed decalcified bone marrow. When aspirate histiocytic iron was minimal or moderate, 14 of 25 B-5 fixed and 7 of 25 zinc-formalin fixed decalcified bone marrow specimens revealed histiocytic iron. Decalcification results in decreased recovery of stainable iron, and where histiocytic iron is minimally or moderately present, B-5 fixation results in greater postdecalcification recovery. There was no significant difference in recovery when larger quantities of histiocytic iron were present prior to the decalcification step.
铁染色常用于通过髂嵴穿刺活检获取的骨髓核心活检标本。由于必须通过用酸进行短暂处理使骨脱钙，所以有可能会损失不确定量的可染色组织细胞铁。开展了一项研究，以确定脱钙是否会导致组织细胞铁的丢失、固定剂的影响以及脱钙骨髓组织中组织细胞铁的恢复情况。骨髓穿刺液用普鲁士蓝进行铁染色。由于穿刺液材料不需要脱钙，所以它作为该研究的对照。对100名成年受试者的100份骨髓活检标本及相应的穿刺液进行了评估。50份骨髓活检标本用含氯化汞的固定剂（B-5）固定，其余50份用锌-福尔马林固定。根据观察到的铁阳性组织细胞分别少于5个、6 - 10个或多于10个，将组织细胞铁分级为少量、中等量或大量。当穿刺液材料中明显存在大量组织细胞铁时，在25份用B-5固定的脱钙骨髓中，有22份至少发现中等量的可染色铁，在25份用锌-福尔马林固定的脱钙骨髓中，有21份发现中等量的可染色铁。当穿刺液中的组织细胞铁为少量或中等量时，在25份用B-5固定的脱钙骨髓标本中，有14份显示有组织细胞铁，在25份用锌-福尔马林固定的脱钙骨髓标本中，有7份显示有组织细胞铁。脱钙会导致可染色铁的回收率降低，并且在组织细胞铁为少量或中等量的情况下，B-5固定会使脱钙后有更高的回收率。在脱钙步骤之前存在大量组织细胞铁时，回收率没有显著差异。
