Gaulier A, Fourcade C, Szekeres G, Pulik M
Service d'Anatomie Cytologie Pathologiques, C. H. Victor Dupouy, Argenteuil, France.
Pathol Res Pract. 1994 Dec;190(12):1149-61. doi: 10.1016/s0344-0338(11)80441-3.
The immunoreactivity of paraffin embedded bone marrow biopsies (BMB) was studied following a one step 20-hour-fixation-decalcification in Lowy formalin mercuric chlorid acid solution which permits excellent histological stainings. Antibodies reactive with myeloid, megakaryocytic, erythroid cells, T and B lymphocytes, mastocytes and metastatic cells were compared. Nearly all antibodies working on paraffin sections were demonstrated on Lowy FMA fixed BMB. Special care was taken to define an optimal working dilution. Trypsinization was not necessary. A slide microwave pre-treatment appeared essential before testing CD20 L26, CD8, CD3, CD34, MB1 Kappa and Lambda antibodies. It was suitable for UCHL1, LN2, CD30 antibodies. The same fixative allowed an m RNA Kappa or Lambda in myeloma and EBER 1 EBV RNAs in HIV lymphoma visualization by in situ hybridization. The safety handling of the toxic mercuric chloride component is discussed.
采用Lowy甲醛-氯化汞酸溶液进行一步20小时固定脱钙处理后，对石蜡包埋的骨髓活检组织（BMB）的免疫反应性进行了研究，该溶液能实现出色的组织学染色。比较了与髓细胞、巨核细胞、红细胞、T和B淋巴细胞、肥大细胞及转移细胞反应的抗体。几乎所有用于石蜡切片的抗体在Lowy FMA固定的BMB上均得到验证。特别注意确定最佳工作稀释度。无需胰蛋白酶处理。在检测CD20 L26、CD8、CD3、CD34、MB1 Kappa和Lambda抗体之前，玻片微波预处理似乎必不可少。它适用于UCHL1、LN2、CD30抗体。相同的固定剂可通过原位杂交使骨髓瘤中的mRNA Kappa或Lambda以及HIV淋巴瘤中的EBER 1 EBV RNA可视化。讨论了有毒氯化汞成分的安全处理问题。
