From The Cole Eye Institute, Cleveland Clinic (H.Z., L.A., I.T., W.J.D., J.B.R.).
From The Cole Eye Institute, Cleveland Clinic (H.Z., L.A., I.T., W.J.D., J.B.R.); Cleveland Clinic Lerner College of Medicine of Case Western Reserve University (W.J.D., J.B.R.); Department of Biomedical Engineering, Case Western Reserve University (W.J.D.), Cleveland, Ohio.
Am J Ophthalmol. 2023 Oct;254:128-140. doi: 10.1016/j.ajo.2023.03.018. Epub 2023 Mar 23.
To characterize focal biomechanical differences between normal, keratoconic, and post-laser vision correction (LVC) corneas using motion-tracking Brillouin microscopy.
Prospective cross-sectional study.
Thirty eyes from 30 patients (10 normal controls [Controls], 10 post-LVC, and 10 stage I or II keratoconus [KC]) had Scheimpflug and motion-tracking Brillouin microscopy imaging using a custom-built device. Mean, maximum (max) and minimum (min) Brillouin shift, spatial standard deviation, and max-min values were compared. Min values were correlated with local Brillouin values at multiple Scheimpflug imaging locations.
Mean (P < .0003), min (P < .00001), spatial standard deviation (P < .01), and max-min (P < .001) were significantly different between the groups. In post hoc pairwise comparisons, the best differentiators for group comparisons were mean (P = .0004) and min (P = .000002) for Controls vs KC, min (P = .0022) and max-min (P = .002) for Controls vs LVC, and mean (P = .0037) and min (P = .0043) for LVC vs KC. Min (area under the receiver operating characteristic = 1.0) and mean (area under the receiver operating characteristic = 0.96) performed well in differentiating Control and KC eyes. Min values correlated best with Brillouin shift values at the thinnest corneal point (r = 0.871, P = .001) and maximum keratometry value identified in the tangential curvature map (r = 0.840, P = .002).
Motion-tracking Brillouin microscopy effectively characterized focal corneal biomechanical alterations in LVC and KC and clearly differentiated these groups from Controls. Primary motion-tracking Brillouin metrics performed well in differentiating groups as compared with basic Scheimpflug metrics, in contrast to previous Brillouin studies, and identified focal changes after LVC where prior Brillouin studies did not.
使用运动跟踪布里渊显微镜描述正常、圆锥角膜和激光视力矫正（LVC）后角膜的局灶生物力学差异。
前瞻性横断面研究。
对 30 名患者（10 名正常对照[对照组]、10 名 LVC 后和 10 名 I 期或 II 期圆锥角膜[KC]患者）的 30 只眼进行 Scheimpflug 和运动跟踪布里渊显微镜成像，使用定制设备。比较平均、最大（max）和最小（min）布里渊频移、空间标准差和 max-min 值。min 值与多个 Scheimpflug 成像位置的局部布里渊值相关。
各组之间的平均（P<0.0003）、最小（P<0.00001）、空间标准差（P<0.01）和 max-min （P<0.001）差异均有统计学意义。在事后两两比较中，用于组间比较的最佳区分指标为对照组与 KC 组的平均（P=0.0004）和 min（P=0.000002），对照组与 LVC 组的 min（P=0.0022）和 max-min（P=0.002），LVC 与 KC 组的平均（P=0.0037）和 min（P=0.0043）。min（受试者工作特征曲线下面积=1.0）和平均（受试者工作特征曲线下面积=0.96）在区分对照组和 KC 眼方面表现良好。min 值与角膜最薄点的布里渊频移值相关性最好（r=0.871，P=0.001），与切线曲率图中最大角膜曲率值相关性最好（r=0.840，P=0.002）。
运动跟踪布里渊显微镜有效地描述了 LVC 和 KC 局灶性角膜生物力学改变，并清楚地区分了这些组与对照组。与之前的布里渊研究相比，主要的运动跟踪布里渊指标在区分组方面表现良好，而基本的 Scheimpflug 指标表现不佳，并且确定了 LVC 后之前的布里渊研究没有发现的局灶性变化。
