Galante E, Caravaggio T, Righetti P G
Biochim Biophys Acta. 1976 Sep 6;442(3):309-15. doi: 10.1016/0005-2787(76)90305-1.
The melting temperature of isoaccepting tRNAfMet is affected by Ampholine. The plot of Tm versus the logarithm of Ampholine concentration shows clearly an increasing effect of Ampholine when the pH changes from 7.4 to 4.2. This result is interpreted as binding of Ampholine to the nucleic acid. The effects of Ampholine have been compared with those of soidum, magnesium and tetraethylene pentamine. Ampholine carrier ampholytes at pH 4.2 bind to tRNA with the same affinity as magnesium; at higher pH values they are less active. An hypothesis for the mechanism of action of Ampholine on nucleic acids during isoelectric focusing is proposed.
同功tRNAfMet的解链温度受两性电解质影响。解链温度（Tm）与两性电解质浓度对数的关系图清楚地表明，当pH从7.4变为4.2时，两性电解质的作用增强。该结果被解释为两性电解质与核酸的结合。已将两性电解质的作用与钠、镁和四乙烯五胺的作用进行了比较。pH 4.2时的两性电解质载体两性离子与tRNA的结合亲和力与镁相同；在较高pH值时它们的活性较低。提出了关于两性电解质在等电聚焦过程中对核酸作用机制的假说。
