人肝碱性磷酸酶的等电聚焦
Isoelectric focusing of human liver alkaline phosphatase.
作者信息
Latner A L, Parsons M E, Skillen A W
出版信息
Biochem J. 1970 Jun;118(2):299-302. doi: 10.1042/bj1180299.
Abstract
- Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of human liver alkaline phosphatase was found to be pH3.9.
摘要
- 描述了以LKB两性电解质为载体两性电解质，在蔗糖密度梯度中对人肝碱性磷酸酶进行等电聚焦的方法。2. 研究了由于两性电解质的螯合特性和pH梯度引起的问题。3. 设计了一种对抗这些影响的重新激活程序，该程序可能可用于其他碱性磷酸酶。4. 发现人肝碱性磷酸酶的等电点为pH3.9。
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