Spuergin P, Walter M, Schiltz E, Deichmann K, Forster J, Mueller H
University Children's Hospital, Freiburg im Breisgau, Germany.
Allergy. 1997 Mar;52(3):293-8. doi: 10.1111/j.1398-9995.1997.tb00993.x.
The allergic potential of alpha-caseins from bovine, ovine, and goat's milk sharing more than 85% identical amino acids was compared. Caseins were purified by anion-exchange chromatography and used for a specific IgE and IgG ELISA with diluted human sera. Sera were from 17 children with immediate-type allergy to cow's milk, from 59 children with atopy but without food allergy, and from 27 healthy children without atopy disease. The sera of cow's milk-allergic children showed a significantly higher IgE and IgG binding to alpha-caseins from all three species than the sera of the other groups. All groups showed an increased antibody binding to bovine alpha-casein compared to the sheep and goat proteins, but the differences were significant only in the groups of atopic children and of healthy controls. Furthermore, inhibition of the IgE binding to bovine alpha-casein with alpha-casein from cow, goat, and sheep revealed that the alpha-casein from these species are highly cross-reactive; on the basis of the small differences in their primary structure. In conclusion, the milk of goat and sheep harbor an allergic potential and is not suitable for the nutrition of milk-allergic patients.
比较了来自牛、羊和山羊奶中氨基酸同源性超过85%的α-酪蛋白的过敏潜力。通过阴离子交换色谱法纯化酪蛋白，并将其用于与稀释的人血清进行特异性IgE和IgG酶联免疫吸附测定。血清来自17名对牛奶有速发型过敏的儿童、59名患有特应性但无食物过敏的儿童以及27名无特应性疾病的健康儿童。与其他组的血清相比，牛奶过敏儿童的血清显示出与所有三种物种的α-酪蛋白的IgE和IgG结合显著更高。与绵羊和山羊蛋白相比，所有组对牛α-酪蛋白的抗体结合均增加，但差异仅在特应性儿童组和健康对照组中显著。此外，用来自牛、山羊和绵羊的α-酪蛋白抑制IgE与牛α-酪蛋白的结合表明，这些物种的α-酪蛋白具有高度交叉反应性；基于它们一级结构的微小差异。总之，山羊奶和绵羊奶具有过敏潜力，不适合牛奶过敏患者的营养。
