Institute of Industrial Science The University of Tokyo 4-6-1 Komaba, Meguro-ku Tokyo 153-8505 Japan.
ChemistryOpen. 2020 May 12;9(5):573-581. doi: 10.1002/open.202000025. eCollection 2020 May.
Artificial receptor-based protein assays have various attractive features such as a long-term stability, a low-cost production process, and the ease of tuning the target specificity. However, such protein sensors are still immature compared with conventional immunoassays. To enhance the application potential of synthetic sensing materials, organic field-effect transistors (OFETs) are some of the suitable platforms for protein assays because of their solution processability, durability, and compact integration. Importantly, OFETs enable the electrical readout of the protein recognition phenomena of artificial receptors on sensing electrodes. Thus, we believe that OFETs functionalized with artificial protein receptors will be a powerful tool for the on-site analyses of target proteins. In this Minireview, we summarize the recent progress of the OFET-based protein assays including the rational design strategies for devices and sensing materials.
基于人工受体的蛋白质分析具有长期稳定性、低成本的生产工艺以及易于调整目标特异性等各种吸引人的特点。然而，与传统免疫测定相比，这些蛋白质传感器仍不够成熟。为了提高合成传感材料的应用潜力，有机场效应晶体管 (OFET) 是蛋白质分析的合适平台之一，因为它们具有溶液加工性、耐用性和紧凑的集成性。重要的是，OFET 可以对传感电极上人工受体的蛋白质识别现象进行电读出。因此，我们相信，用人工蛋白质受体功能化的 OFET 将成为现场分析目标蛋白质的有力工具。在这篇综述中，我们总结了基于 OFET 的蛋白质分析的最新进展，包括器件和传感材料的合理设计策略。
