Fragu P, Kahn E
Equipe de Microscopie Ionique INSERM, Institut Gustave-Roussy, Villejuif France.
Microsc Res Tech. 1997 Feb 15;36(4):296-300. doi: 10.1002/(SICI)1097-0029(19970215)36:4<296::AID-JEMT7>3.0.CO;2-K.
The secondary ion mass spectrometry (SIMS) microscope has opened new fields in biological investigation because of its ability to map chemical elements that are either naturally present in tissue or introduced for diagnostic or therapeutic purposes. In this review, we will describe our attempts to localise and quantify antitumor drugs in histological sections to better evaluate successful early cancer treatment. Detection is dependent on the presence of chemical elements in the drug structure, for example halogens (F, Br, I, At) which are imaged and quantified within the nuclei. Our methodological approach combines the results obtained with ionic and photonic microscopes on serial sections. Thus, the different structures in tumor tissue (blood vessels and cells) can be identified and drug localisation visualized. Using embedded samples, we demonstrate that both fluorine (19F) in 5-Fluorouracil and iodine (127I) in 4'-iododeoxyrubicin can be mapped in human biopsy material obtained after in vivo chemotherapy.
二次离子质谱（SIMS）显微镜因其能够对组织中天然存在的或为诊断或治疗目的而引入的化学元素进行成像，从而在生物学研究中开辟了新领域。在本综述中，我们将描述我们为在组织学切片中定位和定量抗肿瘤药物以更好地评估早期癌症治疗效果所做的尝试。检测取决于药物结构中化学元素的存在，例如卤素（氟、溴、碘、砹），它们可在细胞核内成像并定量。我们的方法结合了在连续切片上使用离子显微镜和光子显微镜获得的结果。因此，肿瘤组织中的不同结构（血管和细胞）可以被识别，药物定位也可以可视化。通过使用包埋样本，我们证明5-氟尿嘧啶中的氟（¹⁹F）和4'-碘阿霉素中的碘（¹²⁷I）都可以在体内化疗后获得的人体活检材料中进行成像。
