甘草酸对免疫介导的细胞毒性的影响。

Effects of glycyrrhizin on immune-mediated cytotoxicity.

作者信息

Yoshikawa M, Matsui Y, Kawamoto H, Umemoto N, Oku K, Koizumi M, Yamao J, Kuriyama S, Nakano H, Hozumi N, Ishizaka S, Fukui H

机构信息

Third Department of Internal Medicine, Nara Medical University, Japan.

出版信息

J Gastroenterol Hepatol. 1997 Mar;12(3):243-8. doi: 10.1111/j.1440-1746.1997.tb00416.x.

DOI:10.1111/j.1440-1746.1997.tb00416.x

PMID:9142643

Abstract

Intravenous administration of glycyrrhizin is known to decrease elevated plasma transaminase levels in patients with chronic viral hepatitis, in which immune-mediated cytotoxicity by cytotoxic T lymphocytes and tumour necrosis factor (TNF)-alpha is considered to play an important pathogenic role. However, the immunological interpretation of the transaminase-lowering action of glycyrrhizin is not known. Studies were performed to elucidate this action immunologically by assessing the effects of glycyrrhizin on immune-mediated cytotoxicity using an antigen-specific murine CD4+ T hybridoma line, which exhibits cytotoxicity against antigen-presenting cells after stimulation with specific antigen, and a murine TNF-alpha-sensitive fibroblast line. Glycyrrhizin inhibited the cytotoxic activity of the T cells against antigen-presenting cells and also suppressed TNF-alpha-induced cytotoxicity in the TNF-alpha-sensitive cell line in vitro. These results suggest that the decrease of elevated transaminase levels by glycyrrhizin in patients with chronic viral hepatitis is mediated in part by inhibition of immune-mediated cytotoxicity against hepatocytes.

摘要

已知静脉注射甘草酸可降低慢性病毒性肝炎患者升高的血浆转氨酶水平，在慢性病毒性肝炎中，细胞毒性T淋巴细胞和肿瘤坏死因子（TNF）-α介导的免疫细胞毒性被认为起重要致病作用。然而，甘草酸降低转氨酶作用的免疫学解释尚不清楚。本研究通过使用抗原特异性小鼠CD4+T杂交瘤细胞系（在受到特异性抗原刺激后对抗原呈递细胞具有细胞毒性）和小鼠TNF-α敏感成纤维细胞系，评估甘草酸对免疫介导细胞毒性的影响，从免疫学角度阐明这一作用。甘草酸在体外抑制T细胞对抗原呈递细胞的细胞毒性活性，并抑制TNF-α敏感细胞系中TNF-α诱导的细胞毒性。这些结果表明，甘草酸降低慢性病毒性肝炎患者升高的转氨酶水平部分是通过抑制针对肝细胞的免疫介导细胞毒性来实现的。