Gilbert E S, Crowley D E
Environmental Toxicology Graduate Program, University of California, Riverside 92521, USA.
Appl Environ Microbiol. 1997 May;63(5):1933-8. doi: 10.1128/aem.63.5.1933-1938.1997.
Plant compounds that induced Arthrobacter sp. strain B1B to cometabolize polychlorinated biphenyls (PCBs) were identified by a screening assay based on the formation of a 4,4'-dichlorobiphenyl ring fission product. A chemical component of spearmint (Mentha spicata), l-carvone, induced Arthrobacter sp. strain B1B to cometabolize Aroclor 1242, resulting in significant degradation of 26 peaks in the mixture, including selected tetra- and pentachlorobiphenyls. Evidence for PCB biodegradation included peak disappearance, formation of a phenylhexdienoate ring fission product, and chlorobenzoate accumulation in the culture supernatant. Carvone was not utilized as a growth substrate and was toxic at concentrations of greater than 500 mg liter-1. Several compounds structurally related to l-carvone, including limonene, p-cymene, and isoprene, also induced cometabolism of PCBs by Arthrobacter sp. strain B1B. A structure-activity analysis showed that chemicals with an unsaturated p-menthane structural motif promoted the strongest cometabolism activity. These data suggest that certain plant-derived terpenoids may be useful for promoting enhanced rates of PCB biodegradation by soil bacteria.
通过基于4,4'-二氯联苯环裂变产物形成的筛选试验，鉴定出了能诱导节杆菌属菌株B1B对多氯联苯（PCBs）进行共代谢的植物化合物。留兰香（薄荷）的一种化学成分——左旋香芹酮，能诱导节杆菌属菌株B1B对氯丹1242进行共代谢，导致混合物中的26个峰显著降解，包括选定的四氯和五氯联苯。多氯联苯生物降解的证据包括峰消失、苯基己二烯酸酯环裂变产物的形成以及培养上清液中氯苯甲酸的积累。香芹酮未被用作生长底物，在浓度大于500毫克/升时具有毒性。几种与左旋香芹酮结构相关的化合物，包括柠檬烯、对伞花烃和异戊二烯，也能诱导节杆菌属菌株B1B对多氯联苯进行共代谢。结构活性分析表明，具有不饱和对薄荷烷结构基序的化学物质促进共代谢活性最强。这些数据表明，某些植物来源的萜类化合物可能有助于提高土壤细菌对多氯联苯的生物降解速率。
