Liguory O, David F, Sarfati C, Schuitema A R, Hartskeerl R A, Derouin F, Modaï J, Molina J M
Division of Infectious Diseases, Hôpital Saint-Louis, Paris, France.
AIDS. 1997 May;11(6):723-6. doi: 10.1097/00002030-199706000-00004.
To study the usefulness of polymerase chain reaction (PCR) for the species identification of microsporidia in stool specimens obtained from HIV-infected patients with Enterocytozoon bieneusi or Encephalitozoon intestinalis infections.
Infectious disease clinic in a university hospital.
Thirty-seven stool specimens from 29 HIV-infected patients with microsporidiosis were tested. The diagnosis of microsporidian infection was made by light microscopy of stool specimens and species identification was made by transmission electron microscopy of duodenal biopsies. Sixty-one stool specimens from 45 HIV-infected patients without microsporidiosis served as controls.
PCR was performed using DNA extracted from stools with two primers sets, one specific for E. bieneusi and one specific for E. intestinalis.
A 1265 base-pair fragment of the small subunit ribosomal RNA (rrs) gene could be amplified from all 31 stool specimens infected with E. bieneusi. In addition, a 930 base-pair fragment of the rrs gene could be amplified from all six stool specimens infected with E. intestinalis. The 61 control stools were negative with both primers.
These results suggest that a PCR based assay using species-specific primers sets can be used successfully for microsporidian species differentiation from stool specimens, thus obviating the need for invasive biopsy procedures.
研究聚合酶链反应（PCR）在从感染肠内微孢子虫或肠脑炎微孢子虫的HIV感染患者粪便标本中进行微孢子虫物种鉴定的实用性。
大学医院的传染病诊所。
对29例感染微孢子虫病的HIV感染患者的37份粪便标本进行检测。通过粪便标本的光学显微镜检查诊断微孢子虫感染，并通过十二指肠活检的透射电子显微镜检查进行物种鉴定。45例未感染微孢子虫病的HIV感染患者的61份粪便标本作为对照。
使用从粪便中提取的DNA，用两组引物进行PCR，一组对肠内微孢子虫特异，另一组对肠脑炎微孢子虫特异。
从所有31份感染肠内微孢子虫的粪便标本中均可扩增出小亚基核糖体RNA（rrs）基因的1265个碱基对片段。此外，从所有6份感染肠脑炎微孢子虫的粪便标本中均可扩增出rrs基因的930个碱基对片段。61份对照粪便标本用两组引物检测均为阴性。
这些结果表明，使用物种特异性引物组的基于PCR的检测方法可成功用于从粪便标本中区分微孢子虫物种，从而无需进行侵入性活检程序。
