通过聚合酶链反应（PCR）检测艾滋病患者标本中的微孢子虫寄生虫肠脑炎微孢子虫。
Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR.
作者信息
Velásquez J N, Carnevale S, Guarnera E A, Labbé J H, Chertcoff A, Cabrera M G, Rodríguez M I
机构信息
Department of Parasitology, National Institute of Microbiology, Dr. Carlos G. Malbrán, Buenos Aires, Argentina.
出版信息
J Clin Microbiol. 1996 Dec;34(12):3230-2. doi: 10.1128/jcm.34.12.3230-3232.1996.
Abstract
Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique rRNA intergenic spacer could be amplified from all samples infected with E. bieneusi, but no amplification was seen by using DNA purified from samples with Septata intestinalis or other parasites and from negative control human cells. These results suggest that the PCR in stool samples may be a useful tool for the diagnosis of intestinal microsporidiosis in patients with AIDS.
摘要
微孢子虫是原生动物寄生虫，可导致感染人类免疫缺陷病毒的患者出现严重的胃肠道疾病。我们评估了一种针对感染比氏肠微孢子虫患者的粪便样本、十二指肠抽吸物和活检标本的聚合酶链反应（PCR）检测方法。从所有感染比氏肠微孢子虫的样本中都能扩增出独特的核糖体RNA基因间隔区的210碱基对DNA片段，但使用从感染肠 septata 或其他寄生虫的样本以及阴性对照人类细胞中纯化的DNA时未观察到扩增。这些结果表明，粪便样本中的PCR可能是诊断艾滋病患者肠道微孢子虫病的有用工具。
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