Yamamoto K, Miura O, Hirosawa S, Miyasaka N
The First Department of Internal Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Japan.
Biochem Biophys Res Commun. 1997 Apr 7;233(1):126-32. doi: 10.1006/bbrc.1997.6415.
Studies of transcriptional activation by interferons and various cytokines have led to the identification of a family of proteins that serve as signal transducers and activators of transcription (STAT). STAT4 is phosphorylated following interleukin (IL)-12 stimulation and is required for IL-12 signal transduction. By immunoprecipitation and PCR amplification, a specific consensus sequence for DNA binding of the STAT4 complex was determined. The binding sequence of the STAT4 complex, (T/A)TTCC(C/G)GGAA(T/A), proved to be palindromic and similar to the IFN-gamma activated site (GAS)-like sequence. The first (T/A) and last (T/A) sites of the consensus sequence were critical for the binding affinity of the STAT4 complex.
对干扰素和各种细胞因子介导的转录激活作用的研究，已促使人们鉴定出一类作为信号转导子和转录激活子（STAT）的蛋白质家族。STAT4在白细胞介素（IL）-12刺激后发生磷酸化，并且是IL-12信号转导所必需的。通过免疫沉淀和PCR扩增，确定了STAT4复合物DNA结合的特定共有序列。结果证明，STAT4复合物的结合序列（T/A）TTCC（C/G）GGAA（T/A）是回文结构，并且类似于IFN-γ激活位点（GAS）样序列。共有序列的第一个（T/A）和最后一个（T/A）位点对STAT4复合物的结合亲和力至关重要。
