Macchia L, Di Paola R, Guerrese M C, Chiechi L M, Tursi A, Caiaffa M F, Haeggstrom J Z
Department of Clinical Immunology and Allergology, University of Bari, Italy.
Biochem Biophys Res Commun. 1997 Apr 17;233(2):496-501. doi: 10.1006/bbrc.1997.6492.
Prostaglandins play an important role in reproduction, particularly in pregnancy and parturition. We investigated the expression of prostaglandin endoperoxide H synthase (PGHS) 1 and 2, key enzymes in prostaglandin biosynthesis, in the human placenta at term. Northern blot analysis of placenta total and poly(A)+ RNA failed to detect significant levels of PGHS-1 mRNA, using a human PGHS-1 specific DNA probe. In contrast, definite PGHS-2 mRNA bands, with a calculated size of about 4.5 kb, were observed in Northern blots of placenta poly(A)+ RNA, using a PGHS-2 specific DNA probe. Moreover, PGHS-1 and PGHS-2 expression was assessed at the translational level by Western blot analysis. Thus, using three highly specific antibodies, we found a selective expression of PGHS-2 immunoreactive protein. These results indicate that PGHS-2 is the PGHS isoform prevalently expressed in the human placenta at term and support the hypothesis that PGHS-2 plays a prominent role in the maintenance of pregnancy, in line with the proposed anti-apoptotic and growth promoting properties of this enzyme.
前列腺素在生殖过程中发挥着重要作用，尤其是在妊娠和分娩过程中。我们研究了足月人胎盘中前列腺素内过氧化物H合酶（PGHS）1和2（前列腺素生物合成中的关键酶）的表达。使用人PGHS - 1特异性DNA探针，对胎盘总RNA和聚腺苷酸加尾（poly(A)+）RNA进行Northern印迹分析，未检测到显著水平的PGHS - 1 mRNA。相反，使用PGHS - 2特异性DNA探针，在胎盘poly(A)+ RNA的Northern印迹中观察到明确的PGHS - 2 mRNA条带，计算大小约为4.5 kb。此外，通过蛋白质印迹分析在翻译水平评估PGHS - 1和PGHS - 2的表达。因此，使用三种高度特异性抗体，我们发现了PGHS - 2免疫反应性蛋白的选择性表达。这些结果表明，PGHS - 2是足月人胎盘中普遍表达的PGHS同工型，并支持PGHS - 2在维持妊娠中起重要作用的假设，这与该酶所提出的抗凋亡和促进生长特性一致。
