Gould T J, Adams C E, Bickford P C
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262, USA.
Neuropharmacology. 1997 Jan;36(1):75-81. doi: 10.1016/s0028-3908(96)00148-7.
The presence of norepinephrine (NE) and NE activated cells, in the deep cerebellar nuclei (DCN) of male F344 rats, was investigated using immunohistochemistry and electrophysiology, during iontophoresis of the beta-adrenergic agonist isoproterenol (ISO). During extracellular electrophysiology, GABA was iontophoretically applied to the cell and ISO was then co-applied in an attempt to modulate the GABAergic inhibition of cell firing in the DCN. Immunohistochemistry was used to detect tyrosine hydroxylase (TH) positive fibers in the DCN. Isoproterenol modulated GABAergic inhibition in 51% of the DCN cells recorded from. In addition, TH-positive fibers that appeared to make contact with DCN cells were found. Therefore, this study demonstrated that functional NE receptors exist in the DCN and NE appears to be present in fibers therein.
利用免疫组织化学和电生理学方法，在β-肾上腺素能激动剂异丙肾上腺素（ISO）离子导入期间，对雄性F344大鼠小脑深部核团（DCN）中去甲肾上腺素（NE）及NE激活细胞的存在情况进行了研究。在细胞外电生理学实验中，将γ-氨基丁酸（GABA）离子导入细胞，随后共同导入ISO，试图调节DCN中细胞放电的GABA能抑制作用。采用免疫组织化学法检测DCN中酪氨酸羟化酶（TH）阳性纤维。异丙肾上腺素对所记录的51%的DCN细胞的GABA能抑制产生了调节作用。此外，还发现了似乎与DCN细胞有接触的TH阳性纤维。因此，本研究表明DCN中存在功能性NE受体，且NE似乎存在于其中的纤维中。
