Canteros C E, Rodero L, Rivas M C, Davel G
Departamento de Micologia, Instituto Nacional de Microbiologia Dr. Carlos Malbran, Buenos Aires, Argentina.
Mycopathologia. 1996;136(1):21-3. doi: 10.1007/BF00436656.
A rapid method to evidence urease activity is described. Urea hydrolysis and consequent production ammonia are detected by a chemical reaction producing a blue phenol compound (indophenol blue). Three hundred and three yeast were tested. Out of 107 urease-positive organisms detected by Christensen's Urea Agar Test (CUAT) 102 were positive by our method. No false negatives were observed by this method when testing 87 Cryptococcus strains. Ths practical screening test for presumptive identification of Cryptococcus neoformans is simple, unaffected by pH changes and requires 15 minutes to be performed.
描述了一种证明脲酶活性的快速方法。通过产生蓝色酚类化合物(靛酚蓝)的化学反应检测尿素水解及随后产生的氨。对303株酵母进行了测试。在通过克里斯滕森尿素琼脂试验(CUAT)检测出的107株脲酶阳性生物体中,有102株通过我们的方法呈阳性。在检测87株隐球菌菌株时,该方法未观察到假阴性。这种用于初步鉴定新型隐球菌的实际筛选试验简单,不受pH变化影响,且只需15分钟即可完成。