哺乳动物精子的DNA荧光测定法。
DNA-fluorometry of mammalian sperm.
作者信息
Zante J, Schumann J, Göhde W, Hacker U
出版信息
Histochemistry. 1977 Oct 3;54(1):1-7. doi: 10.1007/BF00493324.
PMID:914658
Abstract
The Dna-content of sperm and testicular cells was measured by pulse-cytophotometry with high resolution. From flat sperm symmetric and narrow fluorescene distributions were obtained. Enzymatic treatment with papain or pronase and staining with an ethidiumbromide-mithramycin dye solution generate stoichiometric DNA-staining including that of mature sperm with a coefficient of variation below 2%.
摘要
通过高分辨率脉冲细胞光度法测量精子和睾丸细胞的DNA含量。从扁平精子中获得了对称且狭窄的荧光分布。用木瓜蛋白酶或链霉蛋白酶进行酶处理，并用溴化乙锭-光神霉素染料溶液染色，可产生化学计量的DNA染色，包括成熟精子的染色，变异系数低于2%。
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