人类精子的流式细胞术

Flow cytometry of human spermatozoa.

作者信息

Otto F J, Hacker U, Zante J, Schumann J, Göhde W, Meistrich M L

出版信息

Histochemistry. 1979 Jul 11;61(3):249-54. doi: 10.1007/BF00508445.

Abstract

Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes an effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochromes is achieved. Thus reliable and precise measurements of the relative DNA content of human spermatozoa are possible and the two subpopulations of haploid spermatozoa can be distinguished according to the difference in their DNA content.

摘要

给出了用于流式细胞术DNA测量的人类精子制备和染色方法。使用用于二硫键交联还原裂解的试剂和合适的蛋白水解酶，可实现精子染色质的有效解聚以及荧光染料按DNA比例摄取。因此，对人类精子相对DNA含量进行可靠而精确的测量成为可能，并且可以根据单倍体精子DNA含量的差异区分两个亚群。