Lane R W, Yamakoshi J, Kikuchi M, Mizusawa K, Henderson L, Smith M
Kikkoman Corporation Research and Development Division, Chiba, Japan.
Food Chem Toxicol. 1997 Feb;35(2):207-12. doi: 10.1016/s0278-6915(96)00116-0.
Tannase is an acylhydrolase enzyme preparation from Aspergillus oryzae that can be used as a processing aid for the manufacture of cold water-soluble tea beverages. A 91-day oral toxicity study in the rat and a gene mutation study in Salmonella typhimurium were performed to establish the safety of the enzyme preparation for the consumer. General toxicity was low, with no adverse effects observed at the highest dose tested, 1% in the diet. There was no evidence of mutagenic potential with or without metabolic activation. These results, together with knowledge of the production organism and the chemical and microbiological characterization of the enzyme preparation, indicate that tannase can be regarded as safe for its intended use in processing tea.
单宁酶是一种源自米曲霉的酰基水解酶制剂，可用作生产冷水溶性茶饮料的加工助剂。为确定该酶制剂对消费者的安全性，进行了一项为期91天的大鼠口服毒性研究和一项鼠伤寒沙门氏菌基因突变研究。总体毒性较低，在最高测试剂量（饲料中1%）下未观察到不良影响。无论有无代谢活化，均无诱变潜力的证据。这些结果，连同对生产菌株的了解以及酶制剂的化学和微生物特性，表明单宁酶在用于茶叶加工时可被视为安全的。
