Renneberg H, Wennemuth G, Konrad L, Aumüller G
Department of Anatomy and Cell Biology, University of Marburg, Germany.
J Anat. 1997 Apr;190 ( Pt 3)(Pt 3):343-9. doi: 10.1046/j.1469-7580.1997.19030343.x.
An antiserum against secretory vesicles from human seminal fluid (prostasomes) was used to study the localisation and distribution of the respective antigen(s) during prenatal development and pubertal maturation of the human prostate. The crude antiserum stained both secretory and membrane proteins in the adult prostate and other glands, such as pancreas and parotid gland. An immunoaffinity purified fraction from the antiserum selectively reacted with the apical plasma membrane of prostatic epithelium adluminal cells, recognizing a 100 kDa antigen (PMS). Even in the earliest stages of embryonic prostate specimens studied, the adluminal plasma membrane of the epithelial cells from developing glandular anlagen reacted strongly. The occurrence of PMS immunoreactivity in prostatic anlagen was directly correlated with lumen formation. As the antigen is an androgen-independently synthesised membrane protein of the prostate, it may possibly be used as a marker of cell polarity in the normal and pathologically altered prostate.
一种针对人精液分泌小泡（前列腺小体）的抗血清被用于研究在人前列腺产前发育和青春期成熟过程中相应抗原的定位和分布。粗制抗血清可使成年前列腺以及其他腺体（如胰腺和腮腺）中的分泌蛋白和膜蛋白染色。从该抗血清中免疫亲和纯化得到的组分与前列腺上皮腔面细胞的顶端质膜发生选择性反应，识别出一种100 kDa的抗原（PMS）。即使在研究的胚胎前列腺标本的最早阶段，来自发育中的腺原基的上皮细胞的腔面膜也有强烈反应。前列腺原基中PMS免疫反应性的出现与管腔形成直接相关。由于该抗原是前列腺中一种不依赖雄激素合成的膜蛋白，它可能被用作正常和病理改变前列腺中细胞极性的标志物。
