Aumüller G, Seitz J, Bischof W
J Androl. 1983 May-Jun;4(3):183-91. doi: 10.1002/j.1939-4640.1983.tb00748.x.
Acid phosphatase was purified from human prostatic tissue and from seminal plasma. Antisera to antigens from both sources were raised in rabbits. These antisera have been used for immunohistochemical localization of the respective antigens in the prostates of neonatal, infantile, prepubertal, and adult individuals. Immunoreactivity of the prostatic epithelium with the seminal fluid-derived antigen developed progressively in pubertal specimens with increasing age. It was not present in fetal and infantile organs. Antiserum prepared from human prostatic tissue-derived acid phosphatase gave a positive immunoreaction both with stroma and epithelium of the pre- and postpubertal glands. The results give evidence for a clear cut androgen-dependence in the appearance of the acid phosphatase present in semen, which therefore has been identified as secretory. The second antigen is nonsecretory, tissue-bound, nonandrogen dependent, and shares antigenic determinants with leukocyte-derived acid phosphatase.
酸性磷酸酶从人前列腺组织和精浆中纯化得到。用这两种来源的抗原对家兔进行免疫，制备抗血清。这些抗血清已用于免疫组织化学定位新生儿、婴儿、青春期前和成年人前列腺中各自的抗原。青春期标本中，前列腺上皮对精液衍生抗原的免疫反应性随年龄增长而逐渐增强。胎儿和婴儿器官中不存在这种反应。用人前列腺组织衍生的酸性磷酸酶制备的抗血清，对青春期前后腺体的基质和上皮均产生阳性免疫反应。结果表明，精液中酸性磷酸酶的出现明显依赖雄激素，因此被确定为分泌性的。第二种抗原是非分泌性的、与组织结合、不依赖雄激素，且与白细胞衍生的酸性磷酸酶有共同的抗原决定簇。
