人类鱼精蛋白与男性不育症。
Human protamines and male infertility.
作者信息
Khara K K, Vlad M, Griffiths M, Kennedy C R
机构信息
Molecular Medical Research Group, University of Warwick, Biological Sciences, Coventry, UK.
出版信息
J Assist Reprod Genet. 1997 May;14(5):282-90. doi: 10.1007/BF02765830.
PURPOSE
Our purpose was to determine the ratio of protamine 1 to protamine 2 in human spermatozoa and relate it to in vitro fertilization rates (IVF) and standard semen parameters.
METHODS
Couples who had been clinically diagnosed as having male-factor infertility and had undergone IVF treatment were grouped according to IVF rates and pregnancy outcome. Protamines were extracted and separated on acid urea polyacrylamide gels. The P1/P2 ratio correlation with semen parameters and IVF rates was investigated using nonparametric analysis.
RESULTS
The P1/P2 ratio ranged from 0.55 to 1.29 in the control group of patients, who had fertilization rates > or = 50% and had achieved a pregnancy in one IVF cycle. Of the test patients with IVF < 50%, three (14%) had P1/P2 ratios outside the range exhibited by the control group and their sperm possessed large heads.
CONCLUSIONS
P1/P2 ratios were statistically negatively correlated with IVF rates in the control group and with progressive motile sperm concentration in the test group. We do not think that altered P1/P2 ratios are the primary cause of reduced fertilization.
目的
我们的目的是确定人类精子中鱼精蛋白1与鱼精蛋白2的比例，并将其与体外受精率（IVF）和标准精液参数相关联。
方法
将临床诊断为男性因素不育并接受IVF治疗的夫妇根据IVF率和妊娠结局进行分组。提取鱼精蛋白并在酸性尿素聚丙烯酰胺凝胶上进行分离。使用非参数分析研究P1/P2比例与精液参数和IVF率的相关性。
结果
在受精率≥50%且在一个IVF周期内成功妊娠的患者对照组中，P1/P2比例范围为0.55至1.29。在IVF<50%的测试患者中，有三名（14%）的P1/P2比例超出了对照组的范围，并且他们的精子头部较大。
结论
对照组中P1/P2比例与IVF率呈统计学负相关，测试组中与进行性活动精子浓度呈负相关。我们认为P1/P2比例的改变不是受精减少的主要原因。
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