Tejada R I, Mitchell J C, Norman A, Marik J J, Friedman S
Fertil Steril. 1984 Jul;42(1):87-91. doi: 10.1016/s0015-0282(16)47963-x.
A new and practical test for evaluating the fertility of a male subject has been developed. Twenty-eight donors whose semen had induced at least one pregnancy resulting in a normal delivery and 61 patients attending our infertility clinic were studied. Semen smears stained with acridine orange were read on a fluorescence microscope; sperm heads appeared either green (fertile) or red ( nonfertile ). We introduce the concept of an "effective sperm count" which is obtained by multiplying the percentage of green-fluorescing sperm by the actual sperm count. Of our fertile subjects, 27 of 28 (96.4%) exhibited an effective sperm count of greater than or equal to 50 million/ml, while 60 of 61 (98.3%) infertile patients fell below this value. The percent green correlates with neither actual sperm count nor motility, indicating that this test measures a new parameter of male fertility.
一种用于评估男性生育能力的新型实用测试方法已被开发出来。我们研究了28名供体，他们的精液至少使一名女性怀孕并成功顺产，还研究了61名前来我们不孕不育诊所就诊的患者。用吖啶橙染色的精液涂片在荧光显微镜下观察；精子头部呈现绿色（可育）或红色（不育）。我们引入了“有效精子计数”的概念，它是通过将发绿色荧光的精子百分比乘以实际精子计数得到的。在我们的可育受试者中，28人中有27人（96.4%）的有效精子计数大于或等于5000万/毫升，而61名不育患者中有60人（98.3%）低于这个值。绿色精子的百分比与实际精子计数和活力均无关联，这表明该测试衡量的是男性生育能力的一个新参数。
