豚鼠基底动脉平滑肌细胞中细胞内ATP对Ca2+通道刺激的蛋白激酶C需求。
Protein kinase C requirement of Ca2+ channel stimulation by intracellular ATP in guinea-pig basilar artery smooth muscle cells.
作者信息
McHugh D, Beech D J
机构信息
Department of Pharmacology, University of Leeds, UK.
出版信息
J Physiol. 1997 Apr 15;500 ( Pt 2)(Pt 2):311-7. doi: 10.1113/jphysiol.1997.sp022022.
Abstract
- Smooth muscle cells were isolated from guinea-pig basilar artery and conventional whole-cell recordings of Ca2+ channel activity were made at room temperature within 7 h of the isolation procedure. The purpose of the study was to investigate the mechanism of the stimulatory action of intracellular ATP on Ca2+ channels. 2. High (millimolar) concentrations of ATP were needed to produce stimulation of Ca2+ channels, and neither ADP nor AMP mimicked the action of ATP. 3. The ATP effect was not mimicked by stable ATP derivatives (AMP-PNP or AMP-PCP) and was abolished by incubation of cells in non-specific protein kinase inhibitors (staurosporine or H-7) or specific protein kinase C inhibitors (GF109203x, calphostin C or chelerythrine) but not by tyrosine kinase inhibitors (tyrphostin B42 and genistein). 4. The data suggest that ATP-induced stimulation of L-type Ca2+ channels requires functional activity of a protein kinase C isozyme.
摘要
- 从豚鼠基底动脉分离出平滑肌细胞，并在分离过程后的7小时内于室温下进行常规的Ca2+通道活性全细胞记录。本研究的目的是探究细胞内ATP对Ca2+通道的刺激作用机制。2. 需要高（毫摩尔）浓度的ATP才能对Ca2+通道产生刺激作用，ADP和AMP均不能模拟ATP的作用。3. 稳定的ATP衍生物（AMP-PNP或AMP-PCP）不能模拟ATP的作用，用非特异性蛋白激酶抑制剂（星形孢菌素或H-7）或特异性蛋白激酶C抑制剂（GF109203x、钙泊三醇C或白屈菜红碱）孵育细胞可消除ATP的作用，但酪氨酸激酶抑制剂（曲磷胺B42和染料木黄酮）则不能。4. 数据表明，ATP诱导的L型Ca2+通道刺激需要蛋白激酶C同工酶的功能活性。
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1
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J Physiol. 1996 Apr 15;492 ( Pt 2)(Pt 2):359-76. doi: 10.1113/jphysiol.1996.sp021314.
2
Amelioration of vascular dysfunctions in diabetic rats by an oral PKC beta inhibitor.
Science. 1996 May 3;272(5262):728-31. doi: 10.1126/science.272.5262.728.
3
pp60c-src increases voltage-operated calcium channel currents in vascular smooth muscle cells.
Biochem Biophys Res Commun. 1995 Dec 26;217(3):1039-44. doi: 10.1006/bbrc.1995.2874.
4
Regulation of Ca2+ channels by cAMP and cGMP in vascular smooth muscle cells.
Circ Res. 1993 Dec;73(6):1128-37. doi: 10.1161/01.res.73.6.1128.
5
Specificity of protein kinase inhibitor peptides and induction of long-term potentiation.
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4761-5. doi: 10.1073/pnas.91.11.4761.
6
Regulation and modulation of calcium channels in cardiac, skeletal, and smooth muscle cells.
Physiol Rev. 1994 Apr;74(2):365-507. doi: 10.1152/physrev.1994.74.2.365.
7
Protein kinase-C mediates dual modulation of L-type Ca2+ channels in human vascular smooth muscle.
FEBS Lett. 1994 Mar 21;341(2-3):208-12. doi: 10.1016/0014-5793(94)80458-3.
8
Inhibition of omega-conotoxin-sensitive Ca2+ channel currents by internal Mg2+ in cultured rat cerebellar granule neurones.
Pflugers Arch. 1993 Dec;425(5-6):518-27. doi: 10.1007/BF00374880.
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10
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