Horton Y M, Lubbert H, Houslay M D
Molecular Pharmacology Group, University of Glasgow, Scotland, UK.
Mol Membr Biol. 1996 Jan-Mar;13(1):29-31. doi: 10.3109/09687689609160571.
A 483 bp Bgl II/Sal I repeat free fragment was isolated from the vector containing the human serotonin 5-HT(2B) receptor. This fragment contained part of the coding sequence located within the last exon and the entire 3'-non-translated region. It was used to probe, by Southern blots, Pst I digested DNA from a series of 20 rodent-human somatic cell hybrid lines, each of which contained different complements of human chromosomes. This 5-HT(2B) receptor probe showed hybridization to a 2-7 kb Pst I fragment which revealed 100 percent concordance with location of the gene for this 5-HT receptor variant at chromosome 2.
从含有人类血清素5-HT(2B)受体的载体中分离出一个483 bp的Bgl II/Sal I无重复片段。该片段包含位于最后一个外显子内的部分编码序列和整个3'-非翻译区。通过Southern印迹法，用它来探测一系列20个啮齿动物-人类体细胞杂种细胞系经Pst I消化的DNA，每个细胞系含有不同的人类染色体互补体。这个5-HT(2B)受体探针显示与一个2 - 7 kb的Pst I片段杂交，该片段显示出与2号染色体上这个5-HT受体变体基因的位置100%一致。
