Scherer C G, Boylen C W
J Bacteriol. 1977 Nov;132(2):584-9. doi: 10.1128/jb.132.2.584-589.1977.
Cells of Arthrobacter atrocyaneus and A. crystallopoietes, harvested during their exponential phase, were starved in 0.03 M phosphate buffer (pH 7.0) for 28 days. During this time, the cells maintained 90 to 100% viability. Experimental results were similar for both organisms. Total cellular deoxyribonucleic acid was maintained. Measurable degradation rates for deoxyribonucleic acid as determined by radioisotope techniques were not observed, and only during the initial hours of starvation could a synthetic rate be determined. Total ribonucleic acid levels remained stable for the first 24 h of starvation, after which slow, continuous loss of orcinol-reactive material occurred. Synthetic and degradative rates of ribonucleic acid, as determined by radioisotope techniques, dropped quickly at the onset of starvation. Constant basal rates were attained after 24 h. In A. atrocyaneus, total cell protein was degraded continuously from the onset of starvation. In A. crystallopoietes, total cell protein remained stable for the first 24 h, after which slow continuous loss occurred. After 28 days, the total protein per cell was similar for both organisms. In the first week, amino acid pools stabilized at about 50% of the values characteristic of growth. Rates of degradation of protein decreased rapidly for the first 24 h for both organisms, but leveled to a constant basal rate thereafter. Rates of new protein synthesis dropped during the first 24 h and by 48 h achieved a constant basal rate.
在指数生长期收获的深蓝节杆菌和晶状节杆菌细胞，在0.03M磷酸盐缓冲液（pH 7.0）中饥饿培养28天。在此期间，细胞保持90%至100%的活力。两种菌的实验结果相似。细胞总脱氧核糖核酸得以维持。未观察到通过放射性同位素技术测定的脱氧核糖核酸的可测量降解率，且仅在饥饿最初数小时可测定合成率。饥饿的前24小时，核糖核酸总量保持稳定，此后，地衣酚反应性物质缓慢持续损失。通过放射性同位素技术测定，饥饿开始时核糖核酸的合成和降解率迅速下降。24小时后达到恒定的基础速率。在深蓝节杆菌中，从饥饿开始细胞总蛋白就持续降解。在晶状节杆菌中，细胞总蛋白在最初24小时保持稳定，此后缓慢持续损失。28天后，两种菌的每细胞总蛋白相似。在第一周，氨基酸库稳定在生长特征值的约50%。两种菌的蛋白降解率在最初24小时迅速下降，但此后趋于恒定的基础速率。新蛋白合成率在最初24小时下降，到48小时达到恒定的基础速率。
