Ida S
J Biochem. 1977 Sep;82(3):915-8. doi: 10.1093/oxfordjournals.jbchem.a131769.
Assimilatory nitrite reductase was purified 1,700-fold with a yield of 22% from spinach leaves with a procedure involving ammonium sulfate fractionation, DEAE-cellulose and DEAE-Sephadex chromatography, gel filtration and ferredoxin-Sepharose affinity chromatography. The purified enzyme was apparently homogeneous as shown by disc and SDS-gel electrophoresis with a specific activity (mumol NO2-reduced/min/mg protein) of 140.
通过包括硫酸铵分级分离、DEAE-纤维素和DEAE-葡聚糖凝胶色谱、凝胶过滤以及铁氧化还原蛋白-琼脂糖亲和色谱的步骤，从菠菜叶中纯化出同化型亚硝酸还原酶，纯化倍数为1700倍，产率为22%。如圆盘电泳和SDS-凝胶电泳所示，纯化后的酶显然是均一的，比活性（每分钟每毫克蛋白质还原亚硝酸的微摩尔数）为140。
