大肠杆菌噬菌体MS2 A蛋白的序列。II. 胰凝乳蛋白酶消化肽段的分离与序列测定
Sequence of the A-protein of coliphage MS2. II. Isolation and sequence determination of chymotryptic peptides.
作者信息
Vandekerckhove J S, Gielen J G, Van Montagu M C
出版信息
J Biol Chem. 1977 Nov 10;252(21):7761-72.
PMID:914837
Abstract
A total of 94 different peptides have been isolated from chymotryptic digests of underivatized (35 mg) and of reduced, carboxymethylated and citraconylated (48 mg) A-protein. Their amino acid sequences provide information for 375 residues of the total of 393 amino acids of the A-protein polypeptide chain. Abnormal specificity of chymotrypsin in the digestion of citraconylated A-protein was observed.
摘要
从未衍生化的（35毫克）以及还原、羧甲基化和柠康酰化的（48毫克）A蛋白的胰凝乳蛋白酶消化物中总共分离出94种不同的肽。它们的氨基酸序列为A蛋白多肽链393个氨基酸中的375个残基提供了信息。观察到胰凝乳蛋白酶在消化柠康酰化A蛋白时具有异常的特异性。
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