Deeley R G, Udell D S, Burns A T, Gordon J I, Goldberger R F
J Biol Chem. 1977 Nov 25;252(22):7913-5.
Following either primary or secondary stimulation of cockerels with 17beta-estradiol, vitellogenin mRNA begins to accumulate in the liver after about 30 min, reaches a maximum in 3 days, and decays thereafter with a half-life of 30 h. During primary induction, accumulation of vitellogenin mRNA begins at a low rate (50 nucleotides/s/nuclear equivalent of DNA) and after 4 h, shifts to a higher rate (340 nucleotides/s/nuclear equivalent of DNA). In contrast, during secondary induction (administration of estrogen several weeks after the primary response has ceased), accumulation of vitellogenin mRNA begins at the rate of 350 nucleotides/s/nuclear equivalent of DNA and subsequently increases by about 40%. These accumulation rates result in a maximal level of vitellogenin mRNA that is approximately 1.5 times higher during secondary stimulation than that found during primary stimulation. This difference is sufficient to explain the anamnestic response to secondary hormonal stimulation that results in higher levels of circulating vitellogenin in the plasma of the rooster.
用17β-雌二醇对公鸡进行初次或二次刺激后，卵黄蛋白原mRNA在约30分钟后开始在肝脏中积累，3天内达到最大值，之后以30小时的半衰期衰减。在初次诱导期间，卵黄蛋白原mRNA的积累开始速率较低（50个核苷酸/秒/核当量的DNA），4小时后，转变为较高速率（340个核苷酸/秒/核当量的DNA）。相比之下，在二次诱导期间（初次反应停止数周后给予雌激素），卵黄蛋白原mRNA的积累开始速率为350个核苷酸/秒/核当量的DNA，随后增加约40%。这些积累速率导致卵黄蛋白原mRNA的最大水平在二次刺激期间比初次刺激期间高约1.5倍。这种差异足以解释对二次激素刺激的回忆反应，该反应导致公鸡血浆中循环卵黄蛋白原水平升高。
