Burns A T, Deeley R G, Gordon J I, Udell D S, Mullinix K P, Goldberger R F
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1815-9. doi: 10.1073/pnas.75.4.1815.
We have studied the kinetics of vitellogenin mRNA accumulation in rooster liver after a primary injection of 17beta-estradiol. The levels of vitellogenin mRNA have been determined both by hybridization of total cellular RNA to vitellogenin cDNA and by translation of vitellogenin mRNA in a wheat germ cell-free system. The results obtained by both methods of analysis are in good agreement and indicate that vitellogenin mRNA is present in the liver of normal roosters at a level of 0-5 molecules per liver cell and increases in amount during the 3 days following injection of estrogen, reaching a level of almost 6000 molecules per cell at the peak of the response. The level of vitellogenin mRNA declined exponentially during the next 14 days with a half-life of 29 hr, reaching a level of less than 10 molecules per cell at 17 days after injection of the hormone. The levels of vitellogenin mRNA after stimulation with estrogen have been correlated with the in vivo rate of synthesis of the vitellogenin polypeptide. The results indicate that the rate of vitellogenin synthesis is closely correlated with the level of vitellogenin mRNA. On the basis of these findings, we conclude that vitellogenin mRNA does not exist in the liver in an untranslated form after withdrawal from estrogen.
我们研究了初次注射17β-雌二醇后公鸡肝脏中卵黄蛋白原mRNA积累的动力学。通过将总细胞RNA与卵黄蛋白原cDNA杂交以及在麦胚无细胞系统中翻译卵黄蛋白原mRNA来测定卵黄蛋白原mRNA的水平。两种分析方法得到的结果高度一致，表明正常公鸡肝脏中卵黄蛋白原mRNA的水平为每个肝细胞0 - 5个分子，在注射雌激素后的3天内数量增加，在反应高峰期达到每个细胞近6000个分子的水平。在接下来的14天里，卵黄蛋白原mRNA的水平呈指数下降，半衰期为29小时，在注射激素17天后降至每个细胞少于10个分子的水平。雌激素刺激后卵黄蛋白原mRNA的水平与卵黄蛋白原多肽的体内合成速率相关。结果表明，卵黄蛋白原的合成速率与卵黄蛋白原mRNA的水平密切相关。基于这些发现，我们得出结论，在停止使用雌激素后，卵黄蛋白原mRNA在肝脏中不以未翻译的形式存在。
