大鼠组织中水溶性酰基肉碱和肉碱酰基转移酶的定量分析。

Quantitation of water-soluble acylcarnitines and carnitine acyltransferases in rat tissues.

作者信息

Choi Y R, Fogle P J, Clarke P R, Bieber L L

出版信息

J Biol Chem. 1977 Nov 25;252(22):7930-1.

Abstract

The water-soluble acylcarnitines isolated from rat heart, skeletal muscle, liver, and testis have been characterized. The following acyl residues derived from the acylcarnitine fraction were found: acetyl, propionyl, isobutyryl, butyryl, alpha-methylbutyryl, isovaleryl, tiglyl, caproyl, beta-methylcrotonyl and methacrylyl. The amounts of these acylcarnitines in heart, liver, testis and skeletal muscle from fed rats were determined. Acetylcarnitine was the most abundant acylcarnitine; however, appreciable quantities of propionyl-, isobutyryl-, isovaleryl-, and tiglyl-carnitine were found. The levels of carnitine octanyltransferse, carnitine acetyltransferase and carnitine palmityltransferase activities were determined in several tissues. In addition, carnitine isovaleryltransferase and isobutyryltransferase activities were measured in heart, skeletal muscle, liver, testis and kidney. In all instances the specific activity of isobutyryltransferase was similar to the specific activity of acetyltransferase. The results are consistent with the proposal that carnitine is involved in the catabolism of branched-chain amino acids.

摘要

已对从大鼠心脏、骨骼肌、肝脏和睾丸中分离出的水溶性酰基肉碱进行了表征。从酰基肉碱组分中发现了以下酰基残基：乙酰基、丙酰基、异丁酰基、丁酰基、α-甲基丁酰基、异戊酰基、惕格酰基、己酰基、β-甲基巴豆酰基和甲基丙烯酰基。测定了喂食大鼠的心脏、肝脏、睾丸和骨骼肌中这些酰基肉碱的含量。乙酰肉碱是最丰富的酰基肉碱；然而，也发现了相当数量的丙酰基、异丁酰基、异戊酰基和惕格酰基肉碱。测定了几种组织中肉碱辛酰转移酶、肉碱乙酰转移酶和肉碱棕榈酰转移酶的活性水平。此外，还测量了心脏、骨骼肌、肝脏、睾丸和肾脏中的肉碱异戊酰转移酶和异丁酰转移酶的活性。在所有情况下，异丁酰转移酶的比活性与乙酰转移酶的比活性相似。这些结果与肉碱参与支链氨基酸分解代谢的提议一致。