丙二酰辅酶A对大鼠肝脏和脂肪细胞线粒体中显性和潜在肉碱酰基转移酶活性的影响。
The effect of malonyl-CoA on overt and latent carnitine acyltransferase activities in rat liver and adipocyte mitochondria.
作者信息
Saggerson E D, Carpenter C A
出版信息
Biochem J. 1983 Feb 15;210(2):591-7. doi: 10.1042/bj2100591.
Abstract
- Carnitine palmitoyltransferase and carnitine octanoyltransferase activities were measured in mitochondria at various acyl-CoA concentrations before and after sonication, thus permitting assessment of both overt and latent activities. 2. Overt carnitine palmitoyltransferase in liver and adipocyte mitochondria and overt carnitine octanoyltransferase in liver mitochondria were inhibited by malonyl-CoA. None of the latent activities were affected by this metabolite. 3. 5,5'-Dithiobis-(2-nitrobenzoic acid) stimulated latent hepatic carnitine palmitoyltransferase at low [palmitoyl-CoA]. 4. Starvation (24 h) decreased overt carnitine palmitoyltransferase activity in adipocyte mitochondria, but did not alter the sensitivity of this activity to malonyl-CoA.
摘要
- 在超声处理前后，于不同酰基辅酶A浓度下测定线粒体中的肉碱棕榈酰转移酶和肉碱辛酰转移酶活性，从而能够评估显性和潜在活性。2. 丙二酸单酰辅酶A抑制肝脏和脂肪细胞线粒体中的显性肉碱棕榈酰转移酶以及肝脏线粒体中的显性肉碱辛酰转移酶。该代谢产物对任何潜在活性均无影响。3. 在低[棕榈酰辅酶A]浓度下，5,5'-二硫代双-(2-硝基苯甲酸)刺激肝脏潜在肉碱棕榈酰转移酶。4. 饥饿（24小时）降低脂肪细胞线粒体中显性肉碱棕榈酰转移酶活性，但不改变该活性对丙二酸单酰辅酶A的敏感性。
相似文献
1
The effect of malonyl-CoA on overt and latent carnitine acyltransferase activities in rat liver and adipocyte mitochondria.丙二酰辅酶A对大鼠肝脏和脂肪细胞线粒体中显性和潜在肉碱酰基转移酶活性的影响。
Biochem J. 1983 Feb 15;210(2):591-7. doi: 10.1042/bj2100591.
2
Carnitine acyltransferase activities in rat liver and heart measured with palmitoyl-CoA and octanoyl-CoA. Latency, effects of K+, bivalent metal ions and malonyl-CoA.用棕榈酰辅酶A和辛酰辅酶A测定大鼠肝脏和心脏中的肉碱酰基转移酶活性。潜伏性、钾离子、二价金属离子和丙二酰辅酶A的影响。
Biochem J. 1982 Feb 15;202(2):397-405. doi: 10.1042/bj2020397.
3
Effects of fasting and malonyl CoA on the kinetics of carnitine palmitoyltransferase and carnitine octanoyltransferase in intact rat liver mitochondria.禁食和丙二酰辅酶A对完整大鼠肝脏线粒体中肉碱棕榈酰转移酶和肉碱辛酰转移酶动力学的影响。
FEBS Lett. 1981 Sep 28;132(2):166-8. doi: 10.1016/0014-5793(81)81152-0.
4
Interacting effects of L-carnitine and malonyl-CoA on rat liver carnitine palmitoyltransferase.左旋肉碱与丙二酰辅酶A对大鼠肝脏肉碱棕榈酰转移酶的相互作用
Biochem J. 1985 Aug 15;230(1):161-7. doi: 10.1042/bj2300161.
5
Effects of DL-2-bromopalmitoyl-CoA and bromoacetyl-CoA in rat liver and heart mitochondria. Inhibition of carnitine palmitoyltransferase and displacement of [14C]malonyl-CoA from mitochondrial binding sites.DL-2-溴棕榈酰辅酶A和溴乙酰辅酶A对大鼠肝脏和心脏线粒体的影响。对肉碱棕榈酰转移酶的抑制作用以及[14C]丙二酰辅酶A从线粒体结合位点的置换。
Biochem J. 1985 Aug 15;230(1):169-79. doi: 10.1042/bj2300169.
6
Observations on the affinity for carnitine, and malonyl-CoA sensitivity, of carnitine palmitoyltransferase I in animal and human tissues. Demonstration of the presence of malonyl-CoA in non-hepatic tissues of the rat.关于动物和人体组织中肉碱棕榈酰转移酶I对肉碱的亲和力及丙二酰辅酶A敏感性的观察。大鼠非肝脏组织中丙二酰辅酶A存在的证明。
Biochem J. 1983 Jul 15;214(1):21-8. doi: 10.1042/bj2140021.
7
Carnitine acyltransferase activities in rat brain mitochondria. Bimodal distribution, kinetic constants, regulation by malonyl-CoA and developmental pattern.大鼠脑线粒体中的肉碱酰基转移酶活性。双峰分布、动力学常数、丙二酰辅酶A的调节及发育模式。
Biochem J. 1985 Feb 15;226(1):323-30. doi: 10.1042/bj2260323.
8
Reversible sensitization and desensitization of carnitine palmitoyltransferase I to inhibition by malonyl-CoA in isolated rat liver mitochondria. Significance for the mechanism of malonyl-CoA-induced sensitization.肉碱棕榈酰转移酶I在离体大鼠肝脏线粒体中对丙二酰辅酶A抑制作用的可逆性致敏和脱敏。对丙二酰辅酶A诱导致敏机制的意义。
Biochem J. 1983 Sep 15;214(3):1027-30. doi: 10.1042/bj2141027.
9
Hysteretic behaviour of carnitine palmitoyltransferase. The effect of preincubation with malonyl-CoA.肉碱棕榈酰转移酶的滞后行为。丙二酰辅酶A预温育的影响。
Biochem J. 1986 Jun 15;236(3):917-9. doi: 10.1042/bj2360917.
10
The effect of fasting on the activity of liver carnitine palmitoyltransferase and its inhibition by malonyl-CoA.禁食对肝脏肉碱棕榈酰转移酶活性的影响及其被丙二酰辅酶A的抑制作用。
Biochim Biophys Acta. 1981 Sep 24;665(3):628-31. doi: 10.1016/0005-2760(81)90282-4.
引用本文的文献
1
Acute regulation of 5'-AMP-activated protein kinase by long-chain fatty acid, glucose and insulin in rat primary adipocytes.大鼠原代脂肪细胞中长链脂肪酸、葡萄糖和胰岛素对 5'-AMP 激活的蛋白激酶的急性调节作用。
Biosci Rep. 2012 Dec 13;33(1):71-82. doi: 10.1042/BSR20120031.
2
Inhibition by acetyl-CoA of hepatic carnitine acyltransferase and fatty acid oxidation.乙酰辅酶A对肝脏肉碱脂酰转移酶及脂肪酸氧化的抑制作用。
Biochem J. 1983 Nov 15;216(2):499-502. doi: 10.1042/bj2160499.
3
Cycloheximide blocks changes in rat liver carnitine palmitoyltransferase 1 activity in starvation.放线菌酮可阻止饥饿状态下大鼠肝脏肉碱棕榈酰转移酶1活性的变化。
Biochem J. 1984 Nov 15;224(1):201-6. doi: 10.1042/bj2240201.
4
Binding of malonyl-CoA to isolated mitochondria. Evidence for high- and low-affinity sites in liver and heart and relationship to inhibition of carnitine palmitoyltransferase activity.丙二酰辅酶A与分离的线粒体的结合。肝脏和心脏中高亲和力和低亲和力位点的证据及其与肉碱棕榈酰转移酶活性抑制的关系。
Biochem J. 1984 Sep 15;222(3):639-47. doi: 10.1042/bj2220639.
5
Changes in the ability of malonyl-CoA to inhibit carnitine palmitoyltransferase I activity and to bind to rat liver mitochondria during incubation in vitro. Differences in binding at 0 degree C and 37 degrees C with a fixed concentration of malonyl-CoA.体外孵育期间丙二酰辅酶A抑制肉碱棕榈酰转移酶I活性及与大鼠肝脏线粒体结合能力的变化。在0℃和37℃下，固定浓度的丙二酰辅酶A结合情况的差异。
Biochem J. 1984 Sep 1;222(2):335-42. doi: 10.1042/bj2220335.
6
Carnitine acyltransferase activities in rat brain mitochondria. Bimodal distribution, kinetic constants, regulation by malonyl-CoA and developmental pattern.大鼠脑线粒体中的肉碱酰基转移酶活性。双峰分布、动力学常数、丙二酰辅酶A的调节及发育模式。
Biochem J. 1985 Feb 15;226(1):323-30. doi: 10.1042/bj2260323.
7
Carnitine palmitoyltransferase in liver and five extrahepatic tissues in the rat. Inhibition by DL-2-bromopalmitoyl-CoA and effect of hypothyroidism.大鼠肝脏及五种肝外组织中的肉碱棕榈酰转移酶。DL-2-溴棕榈酰辅酶A的抑制作用及甲状腺功能减退的影响。
Biochem J. 1986 May 15;236(1):137-41. doi: 10.1042/bj2360137.
8
Malonyl-CoA binding site and the overt carnitine palmitoyltransferase activity reside on the opposite sides of the outer mitochondrial membrane.丙二酰辅酶A结合位点和明显的肉碱棕榈酰转移酶活性位于线粒体外膜的两侧。
Proc Natl Acad Sci U S A. 1987 Jan;84(2):378-82. doi: 10.1073/pnas.84.2.378.
9
Comparison of triacylglycerol synthesis in rat brown and white adipocytes. Effects of hypothyroidism and streptozotocin-diabetes on enzyme activities and metabolic fluxes.大鼠棕色和白色脂肪细胞中三酰甘油合成的比较。甲状腺功能减退和链脲佐菌素诱导的糖尿病对酶活性和代谢通量的影响。
Biochem J. 1988 Mar 1;250(2):325-33. doi: 10.1042/bj2500325.
10
Development and characterization of a polyclonal antibody against rat liver mitochondrial overt carnitine palmitoyltransferase (CPT I). Distinction of CPT I from CPT II and of isoforms of CPT I in different tissues.抗大鼠肝脏线粒体显性肉碱棕榈酰转移酶（CPT I）多克隆抗体的研制与特性鉴定。CPT I与CPT II的区分以及不同组织中CPT I同工型的区分。
Biochem J. 1992 Mar 1;282 ( Pt 2)(Pt 2):415-21. doi: 10.1042/bj2820415.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
J Biol Chem. 1964 Feb;239:375-80.
3
4
5
Carnitine octanoyltransferase. Evidence for a new enzyme in mitochondria.肉碱辛酰基转移酶。线粒体中一种新酶的证据。
FEBS Lett. 1971 Jan 12;12(3):134-136. doi: 10.1016/0014-5793(71)80051-0.
6
Studies on carnitine palmitoyl transferase: the similar nature of CPTi (inner form) and CPTo (outer form).肉碱棕榈酰转移酶的研究：肉碱棕榈酰转移酶I（内膜形式）和肉碱棕榈酰转移酶II（外膜形式）的相似性质。
Arch Biochem Biophys. 1980 Oct 1;204(1):71-9. doi: 10.1016/0003-9861(80)90008-9.
7
The effect of fasting on the activity of liver carnitine palmitoyltransferase and its inhibition by malonyl-CoA.禁食对肝脏肉碱棕榈酰转移酶活性的影响及其被丙二酰辅酶A的抑制作用。
Biochim Biophys Acta. 1981 Sep 24;665(3):628-31. doi: 10.1016/0005-2760(81)90282-4.
8
Effects of thyroidectomy and starvation on the activity and properties of hepatic carnitine palmitoyltransferase.甲状腺切除术和饥饿对肝脏肉碱棕榈酰转移酶活性及特性的影响。
Biochem J. 1982 Dec 15;208(3):667-72. doi: 10.1042/bj2080667.
9
Sensitivity of carnitine acyltransferase I to malonly-CoA inhibition in isolated rat liver mitochondria is quantitatively related to hepatic malonyl-CoA concentration in vivo.在分离的大鼠肝脏线粒体中，肉碱酰基转移酶I对丙二酰辅酶A抑制作用的敏感性与体内肝脏丙二酰辅酶A浓度存在定量关系。
Biochem J. 1982 Jul 15;206(1):177-9. doi: 10.1042/bj2060177.
10
Sensitivity of brown-adipose-tissue carnitine palmitoyltransferase to inhibition by malonyl-CoA.棕色脂肪组织肉碱棕榈酰转移酶对丙二酰辅酶A抑制作用的敏感性。
Biochem J. 1982 Apr 15;204(1):373-5. doi: 10.1042/bj2040373.
Zotero 插件