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[利用经硫酸化聚阴离子沉淀的血清组分制备某些类脂蛋白水平升高的对照人血清]

[Preparation of control human sera with increased level of certain classes of lipoproteins using serum fractions sedimented with sulfated polyanions].

作者信息

Khaĭdukova I L, Malakhov V N

出版信息

Vopr Med Khim. 1996 Apr-Jun;42(2):159-67.

PMID:9148604
Abstract

Three main classes of human serum lipoproteins (LP), very low density lipoproteins (VLDLP), low density lipoproteins (LDLP), and high density lipoproteins (HDLP) were selectively sedimented by heparin and dextran sulfate in the presence of metal ions. These data were used for the development of a method for preparation of fractions of human serum enriched with certain class of LP. Using isolated fractions serums with increased content of total cholesterol, cholesterol of HDLP and triglycerides were prepared. These fractions may be used as control samples for evaluation of corresponding parameters in native samples.

摘要

人类血清脂蛋白(LP)主要有三类,即极低密度脂蛋白(VLDLP)、低密度脂蛋白(LDLP)和高密度脂蛋白(HDLP),在金属离子存在的情况下,它们可被肝素和硫酸葡聚糖选择性沉淀。这些数据被用于开发一种制备富含特定类别LP的人血清组分的方法。利用分离出的组分制备了总胆固醇、HDLP胆固醇和甘油三酯含量增加的血清。这些组分可用作对照样品,以评估天然样品中的相应参数。

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