从电鳐电器官中分离出纯胆碱能神经末梢。对其代谢特性进行评估。
Isolation of pure cholinergic nerve endings from Torpedo electric organ. Evaluation of their metabolic properties.
作者信息
Morel N, Israel M, Manaranche R, Mastour-Frachon P
出版信息
J Cell Biol. 1977 Oct;75(1):43-55. doi: 10.1083/jcb.75.1.43.
Abstract
Pure cholinergic nerve endings (synaptosomes) were isolated from the electric organ of Torpedo by a rapid procedure. These synaptosomes are approximately 3 micron in diameter. They contain an occasional mitochondrion, numerous synaptic vesicles, and sometimes an active zone is observed. No postynaptic membrane attachment is found. This nerve ending fraction is extremely pure as shown by morphological controls and biochemical data. It is rich in choline acetyltransferase (450 nmol/h per mg protein) and acetylcholine (ACh) (130 nmol/mg protein). The isolated endings retain their cytoplasmic components and they synthesize ACh and are stable in vitro for several hours, as shown by biochemical measurements and morphological analysis.
摘要
通过一种快速方法从电鳐的电器官中分离出了纯胆碱能神经末梢（突触体）。这些突触体直径约为3微米。它们偶尔含有一个线粒体、大量突触小泡，有时还能观察到一个活性区。未发现与突触后膜的附着。如形态学对照和生化数据所示，这个神经末梢部分极其纯净。它富含胆碱乙酰转移酶（每毫克蛋白质450纳摩尔/小时）和乙酰胆碱（ACh）（每毫克蛋白质130纳摩尔）。分离出的末梢保留了其细胞质成分，能合成乙酰胆碱，并且如生化测量和形态学分析所示，在体外能稳定存在数小时。
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