通过高效液相阳离子交换色谱法一步分离主要和稀有核糖核苷及脱氧核糖核苷，用于测定核酸制剂的纯度。

Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations.

作者信息

Breter H J, Seibert G, Zahn R K

出版信息

J Chromatogr. 1977 Oct 21;140(3):251-6. doi: 10.1016/s0021-9673(00)93584-2.

DOI:10.1016/s0021-9673(00)93584-2

Abstract

A method is described for the separation of 13 major and minor ribo- and deoxyribonucleosides in a single chromatographic run using high-performance liquid chromatography on strongly acidic cation-exchange columns. The method proved useful for the routine determination of small amounts of ribonucleic acid impurities in deoxyribonucleic acid preparations and vice versa. About 3% or even less of nucleic acid contamination in a given sample can be easily detected and quantitatively determined under the conditions used.

摘要

本文描述了一种使用高效液相色谱法在强酸性阳离子交换柱上单次色谱运行中分离13种主要和次要的核糖核苷及脱氧核糖核苷的方法。该方法被证明可用于常规测定脱氧核糖核酸制剂中少量的核糖核酸杂质，反之亦然。在所用条件下，可以轻松检测并定量测定给定样品中约3%甚至更少的核酸污染。

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Single-step separation of major and rare ribonucleosides and deoxyribonucleosides by high-performance liquid cation-exchange chromatography for the determination of the purity of nucleic acid preparations.通过高效液相阳离子交换色谱法一步分离主要和稀有核糖核苷及脱氧核糖核苷，用于测定核酸制剂的纯度。

J Chromatogr. 1977 Oct 21;140(3):251-6. doi: 10.1016/s0021-9673(00)93584-2.

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Separation of bases, ribonucleosides and deoxyribonucleosides by anion-exclusion and partition chromatography on cation-exchange resin: application to the assay of ribonucleotide reductase, deaminase and nucleosidase.基于阳离子交换树脂，通过阴离子排斥和分配色谱法分离碱基、核糖核苷和脱氧核糖核苷：在核糖核苷酸还原酶、脱氨酶和核苷酶测定中的应用

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8

High-performance liquid chromatographic determination of purine and pyrimidine bases, ribonucleosides, deoxyribonucleosides and cyclic ribonucleotides in biological fluids.高效液相色谱法测定生物体液中的嘌呤和嘧啶碱基、核糖核苷、脱氧核糖核苷及环核苷酸。

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Separation of nucleosides on polyacrylamide gel columns: further developments.核苷在聚丙烯酰胺凝胶柱上的分离：进一步进展

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Rapid separation of DNA constituents, bases, nucleosides and nucleotides, under the same chromatographic conditions using high-performance liquid chromatography with a reversed-phase column.在相同色谱条件下，使用反相柱的高效液相色谱法对DNA成分、碱基、核苷和核苷酸进行快速分离。

J Chromatogr. 1979 Mar 1;162(3):319-26. doi: 10.1016/s0378-4347(00)81518-2.

引用本文的文献

1

Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA.反相高效液相色谱法定量测定DNA中的主要和修饰脱氧核糖核苷。

Nucleic Acids Res. 1980 Oct 24;8(20):4763-76. doi: 10.1093/nar/8.20.4763.

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