Hara S, Hayashi S
J Chromatogr. 1977 Nov 11;142:689-703. doi: 10.1016/s0021-9673(01)92077-1.
For systematization of the correlation between the chemical structures of solutes and retention behaviour in liquid column chromatography, the retention volumes of 43 modified steroids on silica and chemically bonded reversed-phase columns were examined using various binary solvent systems. Retention parameters for the functional groups of the steroids were calculated according to Martin's additive rule. By comparing these values obtained on normal and reversed-phase columns, characteristic features of both packings with regard to solute structures and the solvent systems were elaborated.
为了使溶质的化学结构与液相柱色谱中的保留行为之间的相关性系统化，使用各种二元溶剂体系研究了43种改性甾体在硅胶柱和化学键合反相柱上的保留体积。根据马丁加和规则计算甾体官能团的保留参数。通过比较在正相柱和反相柱上获得的这些值，阐述了两种固定相在溶质结构和溶剂体系方面的特征。
