采用液相色谱法测定血浆中氯米帕明和去甲氯米帕明的含量。
Determination of clomipramine and desmethylclomipramine in plasma by means of liquid chromatography.
作者信息
Westenberg H G, Drenth B F, de Zeeuw R A, de Cuyper H, van Praag H M, Korf J
出版信息
J Chromatogr. 1977 Nov 11;142:725-33. doi: 10.1016/s0021-9673(01)92080-1.
A method is presented for the determination of clomipramine and its major metabolite desmethylclomipramine in plasma. After extraction with n-hexane, the components are separated by high performance liquid-solid chromatography on silica gel and detected with a UV detector. The detection limits are 2 ng/ml for clomipramine and 10 ng/ml for desmethylclomipramine. Recoveries from plasma exceed 95% for both drugs. In routine analysis, 30-40 samples can be handled in one day. The practical use of the method is shown in plasma concentration-time curves after oral and intramuscular administration of clomipramine.
本文介绍了一种测定血浆中氯米帕明及其主要代谢物去甲氯米帕明的方法。用正己烷萃取后，通过硅胶柱高效液相色谱法分离各成分，并用紫外检测器进行检测。氯米帕明的检测限为2 ng/ml，去甲氯米帕明的检测限为10 ng/ml。两种药物在血浆中的回收率均超过95%。在常规分析中，一天可处理30 - 40个样本。该方法的实际应用通过口服和肌肉注射氯米帕明后的血浆浓度 - 时间曲线得以展示。
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