Diquet B, Thomaré P, Bocquentin M, Diviné C
Département de Pharmacologie Clinique, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
Biomed Chromatogr. 1993 Mar-Apr;7(2):59-63. doi: 10.1002/bmc.1130070202.
A method has been developed for the quantitative analysis of clomipramine and its major metabolite desmethylclomipramine in plasma, using normal phase chromatography with UV detection at 254 nm. This rapid (6 min) and highly sensitive methodology (detection limits 0.5 ng/mL and 2 ng/mL for clomipramine and desmethylclomipramine, respectively; S/N = 3, 0.001 aufs) allows pharmacokinetic studies and drug monitoring of the two compounds. Using the described methodology we report an application which involved 10 depressed inpatients.
已开发出一种利用正相色谱法在254nm处进行紫外检测来定量分析血浆中氯米帕明及其主要代谢物去甲氯米帕明的方法。这种快速（6分钟）且高度灵敏的方法（氯米帕明和去甲氯米帕明的检测限分别为0.5ng/mL和2ng/mL；信噪比=3，0.001aufs）可用于这两种化合物的药代动力学研究和药物监测。使用所描述的方法，我们报告了一项涉及10名抑郁症住院患者的应用。
