来自拟南芥的一个新型香叶基香叶基焦磷酸合酶基因在大肠杆菌中的克隆及功能表达
Cloning and functional expression of a novel geranylgeranyl pyrophosphate synthase gene from Arabidopsis thaliana in Escherichia coli.
作者信息
Zhu X F, Suzuki K, Okada K, Tanaka K, Nakagawa T, Kawamukai M, Matsuda K
机构信息
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
出版信息
Plant Cell Physiol. 1997 Mar;38(3):357-61. doi: 10.1093/oxfordjournals.pcp.a029174.
A gene encoding a novel geranylgeranyl pyrophosphate (GGPP) synthase from Arabidopsis thaliana has been identified and termed GGPS5. The gene has been sequenced and expressed in Escherichia coli. The deduced amino acid sequence showed 64.5% and 57.5% identify with a putative GGPP synthase from Arabidopsis and Capsicum annuum, respectively. GGPP enzymatic activity was detected in E. coli cells expressing the GGPS5 gene in two different ways. One was the direct measurement of GGPP synthase activity in cell extracts and the other was the yellow color production of cells when the GGPS5 gene was co-expressed with crtB, crtI, crtY and crtZ genes derived from Erwinia uredovora.
已从拟南芥中鉴定出一个编码新型香叶基香叶基焦磷酸（GGPP）合酶的基因，并将其命名为GGPS5。该基因已测序并在大肠杆菌中表达。推导的氨基酸序列与拟南芥和辣椒中假定的GGPP合酶分别有64.5%和57.5%的一致性。以两种不同方式在表达GGPS5基因的大肠杆菌细胞中检测到了GGPP酶活性。一种是直接测量细胞提取物中GGPP合酶的活性，另一种是当GGPS5基因与源自丁香假单胞菌的crtB、crtI、crtY和crtZ基因共表达时细胞产生黄色。
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