Nakamura Y, Sakakibara Y, Kobayashi H, Kasamo K
Molecular Function Laboratory, National Food Research Institute, Ibaraki, Japan.
Plant Cell Physiol. 1997 Mar;38(3):371-4. doi: 10.1093/oxfordjournals.pcp.a029177.
We report the rapid and functional reconstitution of H(+)-pyrophosphatase (H(+)-PPase) from the tonoplast of cultured rice (Oryza sativa L.) cells to proteoliposomes. The CHAPS-solubilized H(+)-PPase was incorporated into liposomes by gel-filtration. Both the activities of PPi-hydrolysis and H(+)-pumping were influenced by the lipid-protein ratio and cholesterol.
我们报道了从培养的水稻（Oryza sativa L.）细胞液泡膜中提取的H⁺-焦磷酸酶（H⁺-PPase）快速且功能性地重组到蛋白脂质体中。通过凝胶过滤将CHAPS增溶的H⁺-PPase整合到脂质体中。焦磷酸水解和H⁺泵浦活性均受脂-蛋白比例和胆固醇的影响。
