Felden B, Himeno H, Muto A, Atkins J F, Gesteland R F
Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA.
Biochimie. 1996;78(11-12):979-83. doi: 10.1016/s0300-9084(97)86720-x.
A secondary structure of Escherichia coli 10Sa RNA (tmRNA) recently proposed on the basis of a variety of chemical and enzymatic probing data combined with phylogenetic analysis (Felden et al, in press), indicates a highly folded structure. Several long-range interactions including pseudoknots are proposed based on comparative analysis of 10 tmRNA genes. Whereas most of the probing data support these predicted secondary structures, several atypical reactivities in specific domains of the molecule suggest structural dynamics, perhaps relating to the complex functions of the molecule as both tRNA and mRNA. The structure of tmRNA has three modular units; a tRNA-like domain, an mRNA-like domain and an intricate connecting unit probably responsible for correct orientation of the two functional parts of the molecule.
最近，基于各种化学和酶促探测数据并结合系统发育分析提出的大肠杆菌10Sa RNA（转运信使RNA）二级结构（费尔登等人，即将发表）表明其结构高度折叠。基于对10个转运信使RNA基因的比较分析，提出了包括假结在内的几种长程相互作用。虽然大多数探测数据支持这些预测的二级结构，但该分子特定结构域中的一些非典型反应性表明存在结构动力学，这可能与该分子作为tRNA和mRNA的复杂功能有关。转运信使RNA的结构有三个模块化单元；一个类似tRNA的结构域、一个类似mRNA的结构域和一个复杂的连接单元，该连接单元可能负责分子两个功能部分的正确定向。
